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Literature summary for 3.4.21.63 extracted from
- Purification and characterization of extracellular proteinases of Aspergillus oryzae (1975), Appl. Microbiol., 30, 507-513.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| sulfhydryl compounds | accelerate the action | Aspergillus oryzae |  |
General Stability
| General Stability | Organism |
|---|---|
| Enzyme is unstable in 8 M urea, overnight at 30°C | Aspergillus oryzae |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Ag+ | - |
Aspergillus oryzae | |
| DFP | - |
Aspergillus oryzae | |
| Fe2+ | - |
Aspergillus oryzae | |
| Fe3+ | - |
Aspergillus oryzae | |
| Hg2+ | - |
Aspergillus oryzae | |
| N-Chlorosuccinimide | - |
Aspergillus oryzae | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | - |
Aspergillus oryzae |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Cu2+ | activation | Aspergillus oryzae | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 35000 | - |
Aspergillus oryzae, gel filtration | Aspergillus oryzae |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Aspergillus oryzae | - |
EI212 | - |
| Aspergillus oryzae EI212 | - |
EI212 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| EI212 | Aspergillus oryzae |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Aspergillus oryzae |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| casein + H2O | - |
Aspergillus oryzae | hydrolyzed casein | - |
? | |
| casein + H2O | - |
Aspergillus oryzae EI212 | hydrolyzed casein | - |
? | |
| Gelatin + H2O | - |
Aspergillus oryzae | Hydrolyzed gelatin | - |
? | |
| Gelatin + H2O | - |
Aspergillus oryzae EI212 | Hydrolyzed gelatin | - |
? | |
| Hemoglobin + H2O | - |
Aspergillus oryzae | Hydrolyzed hemoglobin | - |
? | |
| Hemoglobin + H2O | - |
Aspergillus oryzae EI212 | Hydrolyzed hemoglobin | - |
? | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 10 | - |
casein, hemoglobin | Aspergillus oryzae |
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