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Literature summary for 3.4.21.63 extracted from
- Alkaline proteinases from Aspergillus (1970), Methods Enzymol., 19, 581-591.
No PubMed abstract available
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| DFP | - |
Aspergillus flavus |  |
| DFP | - |
Aspergillus oryzae | |
| DFP | - |
Aspergillus sydowii | |
| additional information | cysteine; EDTA; KCN; not: sulfhydryl reagents; PCMB | Aspergillus flavus | |
| additional information | cysteine; EDTA; KCN; not: sulfhydryl reagents; PCMB | Aspergillus oryzae | |
| additional information | cysteine; EDTA; KCN; not: sulfhydryl reagents; PCMB | Aspergillus sydowii | |
| N-bromosuccinimide | - |
Aspergillus flavus | |
| N-bromosuccinimide | - |
Aspergillus oryzae | |
| N-bromosuccinimide | - |
Aspergillus sydowii | |
| Potato inhibitor | - |
Aspergillus oryzae | |
| Potato inhibitor | - |
Aspergillus sydowii |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| extracellular | - |
Aspergillus flavus | - |
- |
| extracellular | - |
Aspergillus oryzae | - |
- |
| extracellular | - |
Aspergillus sydowii | - |
- |
| extracellular | - |
Aspergillus sojae | - |
- |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 18000 | - |
Aspergillus flavus | Aspergillus flavus |
| 25500 | - |
Aspergillus sojae | Aspergillus sojae |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Aspergillus flavus | - |
- |
- |
| Aspergillus oryzae | - |
- |
- |
| Aspergillus sojae | - |
- |
- |
| Aspergillus sydowii | - |
- |
- |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| additional information | Aspergillus sojae enzyme contains no sugar, Aspergillus flavus alkaline proteinase contains 1 mol ribose per mol of protein | Aspergillus flavus |
| additional information | Aspergillus sojae enzyme contains no sugar, Aspergillus flavus alkaline proteinase contains 1 mol ribose per mol of protein | Aspergillus sojae |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Aspergillus oryzae |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Aspergillus flavus |
| additional information | - |
- |
Aspergillus oryzae |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| Hemoglobin + H2O | - |
Aspergillus flavus | Hydrolyzed hemoglobin | - |
? | |
| Hemoglobin + H2O | - |
Aspergillus oryzae | Hydrolyzed hemoglobin | - |
? | |
| Hemoglobin + H2O | - |
Aspergillus sydowii | Hydrolyzed hemoglobin | - |
? | |
| Hemoglobin + H2O | - |
Aspergillus sojae | Hydrolyzed hemoglobin | - |
? | |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 35 | - |
pH 7.5, highest stability | Aspergillus sydowii |
| 55 | - |
pH 7.5, 10 min, complete inactivation after incubation in 0.01 M sodium phosphate, Aspergillus sydowi | Aspergillus sydowii |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| additional information | - |
pI: 5.1 (Aspergillus sojae) | Aspergillus sojae |
| 9 | 10 | Aspergillus sojae | Aspergillus sojae |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
35°C, highest stability | Aspergillus sydowii |
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Release 2023.1 (January 2023)
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