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Literature summary for 3.4.21.63 extracted from

  • Morihara, K.; Oka, T.; Tsuzuki, H.
    Comparative study of various serine alkaline proteinases from microorganisms. Esterase activity against N-acylated peptide ester substrates (1974), Arch. Biochem. Biophys., 165, 72-79.
    View publication on PubMed
Search for more literature for 3.4.21.63

Inhibitors

Inhibitors Comment Organism Structure
Benzyloxycarbonyl-Ala-Gly-PheCH2Cl
-
 Aspergillus melleus
Benzyloxycarbonyl-Ala-Gly-PheCH2Cl
-
 Aspergillus sojae

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information
-
 Aspergillus sojae
additional information
-
 additional information
-
 Aspergillus melleus
4.7
-
 acetyl-(Ala)4 methyl ester Aspergillus melleus Aspergillus melleus
6.7
-
 acetyl-(Ala)3 methyl ester Aspergillus melleus Aspergillus melleus
6.7
-
 acetyl-(Ala)3 methyl ester acetyl-(Ala)2-Phe methyl ester, Aspergillus sojae Aspergillus sojae
7
-
 acetyl-(Ala)4 methyl ester Aspergillus sojae Aspergillus sojae
13.5
-
 Acetyl-Ala-Phe methyl ester Aspergillus sojae Aspergillus sojae
23
-
 Acetyl-Phe methyl ester Aspergillus sojae Aspergillus sojae
32.1
-
 acetyl-(Ala)3 methyl ester Aspergillus sojae Aspergillus sojae
44.5
-
 acetyl-(Ala)2 methyl ester Aspergillus sojae Aspergillus sojae
68.5
-
 acetyl-(Ala)2 methyl ester Aspergillus melleus Aspergillus melleus
96.8
-
 Acetyl-Ala methyl ester Aspergillus sojae Aspergillus sojae
194
-
 acetyl-(Ala)4 methyl ester Aspergillus melleus Aspergillus melleus

Organism

Organism UniProt Comment Textmining
Aspergillus melleus
-
-
-
Aspergillus sojae
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Acetyl-Ala methyl ester + H2O
-
 Aspergillus sojae Acetyl-Ala + methanol
-
 ?
Acetyl-Ala methyl ester + H2O
-
 Aspergillus melleus Acetyl-Ala + methanol
-
 ?
Acetyl-Ala-Ala methyl ester + H2O
-
 Aspergillus sojae Acetyl-Ala-Ala + methanol
-
 ?
Acetyl-Ala-Ala methyl ester + H2O
-
 Aspergillus melleus Acetyl-Ala-Ala + methanol
-
 ?
Acetyl-Ala-Ala-Ala methyl ester + H2O
-
 Aspergillus sojae Acetyl-Ala-Ala-Ala + methanol
-
 ?
Acetyl-Ala-Ala-Ala methyl ester + H2O
-
 Aspergillus melleus Acetyl-Ala-Ala-Ala + methanol
-
 ?
Acetyl-Ala-Ala-Ala-Ala methyl ester + H2O
-
 Aspergillus sojae Acetyl-Ala-Ala-Ala-Ala + methanol
-
 ?
Acetyl-Ala-Ala-Ala-Ala methyl ester + H2O
-
 Aspergillus melleus Acetyl-Ala-Ala-Ala-Ala + methanol
-
 ?
Acetyl-Ala-Ala-Phe methyl ester + H2O
-
 Aspergillus sojae Acetyl-Ala-Ala-Phe + methanol
-
 ?
Acetyl-Ala-Ala-Phe methyl ester + H2O
-
 Aspergillus melleus Acetyl-Ala-Ala-Phe + methanol
-
 ?
Acetyl-Ala-Phe methyl ester + H2O
-
 Aspergillus sojae Acetyl-Ala-Phe + methanol
-
 ?
Acetyl-Ala-Phe methyl ester + H2O
-
 Aspergillus melleus Acetyl-Ala-Phe + methanol
-
 ?
Acetyl-Phe methyl ester + H2O
-
 Aspergillus sojae Acetyl-Phe + methanol
-
 ?
Acetyl-Phe methyl ester + H2O
-
 Aspergillus melleus Acetyl-Phe + methanol
-
 ?
Benzyloxycarbonyl-Ala-Ala-Lys methyl ester + H2O
-
 Aspergillus sojae Benzyloxycarbonyl-Ala-Ala-Lys + methanol
-
 ?
Benzyloxycarbonyl-Ala-Ala-Lys methyl ester + H2O
-
 Aspergillus melleus Benzyloxycarbonyl-Ala-Ala-Lys + methanol
-
 ?
Benzyloxycarbonyl-Ala-Lys methyl ester + H2O
-
 Aspergillus sojae Benzyloxycarbonyl-Ala-Lys + methanol
-
 ?
Benzyloxycarbonyl-Ala-Lys methyl ester + H2O
-
 Aspergillus melleus Benzyloxycarbonyl-Ala-Lys + methanol
-
 ?
Benzyloxycarbonyl-Gly-Lys methyl ester + H2O
-
 Aspergillus sojae Benzyloxycarbonyl-Gly-Lys + methanol
-
 ?
Benzyloxycarbonyl-Gly-Lys methyl ester + H2O
-
 Aspergillus melleus Benzyloxycarbonyl-Gly-Lys + methanol
-
 ?
Benzyloxycarbonyl-Leu-Lys methyl ester + H2O
-
 Aspergillus sojae Benzyloxycarbonyl-Leu-Lys + methanol
-
 ?
Benzyloxycarbonyl-Leu-Lys methyl ester + H2O
-
 Aspergillus melleus Benzyloxycarbonyl-Leu-Lys + methanol
-
 ?
Benzyloxycarbonyl-Lys methyl ester + H2O
-
 Aspergillus sojae Benzyloxycarbonyl-Lys + methanol
-
 ?
Benzyloxycarbonyl-Lys methyl ester + H2O
-
 Aspergillus melleus Benzyloxycarbonyl-Lys + methanol
-
 ?
Benzyloxycarbonyl-Phe-Lys methyl ester + H2O
-
 Aspergillus sojae Benzyloxycarbonyl-Phe-Lys + methanol
-
 ?
Benzyloxycarbonyl-Phe-Lys methyl ester + H2O
-
 Aspergillus melleus Benzyloxycarbonyl-Phe-Lys + methanol
-
 ?
additional information the esterase activity is markedly promoted by elongating the peptide chain from P1 to P2 or P3 with alanine, irrespectively of the kind of the amino acid residue at the P1-position Aspergillus sojae ?
-
 ?
additional information the esterase activity is markedly promoted by elongating the peptide chain from P1 to P2 or P3 with alanine, irrespectively of the kind of the amino acid residue at the P1-position Aspergillus melleus ?
-
 ?
additional information leucine or phenylalanine is most efficient at the P2-position Aspergillus sojae ?
-
 ?
additional information leucine or phenylalanine is most efficient at the P2-position Aspergillus melleus ?
-
 ?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
28.3
-
 Acetyl-Ala methyl ester Aspergillus melleus Aspergillus melleus
28.9
-
 Acetyl-Ala methyl ester Aspergillus sojae Aspergillus sojae
1150
-
 acetyl-(Ala)2 methyl ester Aspergillus sojae Aspergillus sojae
1540
-
 acetyl-(Ala)3 methyl ester Aspergillus melleus Aspergillus melleus
1970
-
 acetyl-(Ala)4 methyl ester Aspergillus melleus Aspergillus melleus
2000
-
 acetyl-(Ala)2 methyl ester Aspergillus melleus Aspergillus melleus
2090
-
 acetyl-(Ala)4 methyl ester Aspergillus sojae Aspergillus sojae
3720
-
 acetyl-(Ala)3 methyl ester Aspergillus sojae Aspergillus sojae