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Literature summary for 3.4.21.62 extracted from

  • Wong, W.; Wijeyewickrema, L.C.; Kennan, R.M.; Reeve, S.B.; Steer, D.L.; Reboul, C.; Smith, A.I.; Pike, R.N.; Rood, J.I.; Whisstock, J.C.; Porter, C.J.
    The S1 pocket of a bacterial derived subtilisin-like protease underpins effective tissue destruction (2011), J. Biol. Chem., 286, 42180-42187.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.4.21.62

Crystallization (Commentary)

Crystallization (Comment) Organism
data reveal that that the S1 pocket of BprB is less hydrophobic but bigger than that of basic protease BprV (sequence homologue) Dichelobacter nodosus
data reveal that that the S1 pocket of BprV is more hydrophobic but smaller than that of basic protease BprB Dichelobacter nodosus

Protein Variants

Protein Variants Comment Organism
D180G mutant using substrate N-methoxysuccinyl-Ala-Ala-Pro-Val-4-nitroanilide Km similar to wild-type, kcat value enhanced, kcat/Km value enhanced by 4% Dichelobacter nodosus
D180G/D182G using substrate N-methoxysuccinyl-Ala-Ala-Pro-Val-4-nitroanilide Km similar to wild-type, kcat value highly enhanced, kcat/Km value enhanced by 33% Dichelobacter nodosus
D182G using substrate N-methoxysuccinyl-Ala-Ala-Pro-Val-4-nitroanilide Km similar to wild-type, kcat value enhanced, kcat/Km value enhanced by 27% Dichelobacter nodosus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.13
-
 N-methoxysuccinyl-Ala-Ala-Pro-Val-4-nitroanilide pH 8.5, 37°C Dichelobacter nodosus
3.74
-
 N-methoxysuccinyl-Ala-Ala-Pro-Val-4-nitroanilide pH 8.5, 37°C, mutant D182G Dichelobacter nodosus
3.84
-
 N-methoxysuccinyl-Ala-Ala-Pro-Val-4-nitroanilide pH 8.5, 37°C, wild-type Dichelobacter nodosus
4.02
-
 N-methoxysuccinyl-Ala-Ala-Pro-Val-4-nitroanilide pH 8.5, 37°C, mutant D180G/D182G Dichelobacter nodosus
4.28
-
 N-methoxysuccinyl-Ala-Ala-Pro-Val-4-nitroanilide pH 8.5, 37°C, mutant D180G Dichelobacter nodosus

Organism

Organism UniProt Comment Textmining
Dichelobacter nodosus
-
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
 the virulent protease BprV is more efficient than its sequence homolog BprB Dichelobacter nodosus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
human fibronectin + H2O
-
 Dichelobacter nodosus ?
-
 ?
insoluble elastin + H2O
-
 Dichelobacter nodosus ?
-
 ?
Keratin + H2O
-
 Dichelobacter nodosus ?
-
 ?
additional information enzymes prefers cleaving after hydrophobic residues (and in particular P1 leucine) Dichelobacter nodosus ?
-
 ?
N-methoxysuccinyl-Ala-Ala-Pro-Val-4-nitroanilide + H2O
-
 Dichelobacter nodosus N-methoxysuccinyl-Ala-Ala-Pro-Val + 4-nitroaniline
-
 ?

Synonyms

Synonyms Comment Organism
BprB
-
 Dichelobacter nodosus
BprV
-
 Dichelobacter nodosus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
 assay with human fibronectin Dichelobacter nodosus
37
-
 assay with N-methoxysuccinyl-Ala-Ala-Pro-Val-4-nitroanilide Dichelobacter nodosus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.0064
-
 N-methoxysuccinyl-Ala-Ala-Pro-Val-4-nitroanilide pH 8.5, 37°C Dichelobacter nodosus
0.0074
-
 N-methoxysuccinyl-Ala-Ala-Pro-Val-4-nitroanilide pH 8.5, 37°C, mutant D180G Dichelobacter nodosus
0.0079
-
 N-methoxysuccinyl-Ala-Ala-Pro-Val-4-nitroanilide pH 8.5, 37°C, mutant D182G Dichelobacter nodosus
0.0089
-
 N-methoxysuccinyl-Ala-Ala-Pro-Val-4-nitroanilide pH 8.5, 37°C Dichelobacter nodosus
0.0089
-
 N-methoxysuccinyl-Ala-Ala-Pro-Val-4-nitroanilide pH 8.5, 37°C, mutant D180G/D182G Dichelobacter nodosus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.5
-
 assay at Dichelobacter nodosus

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.67
-
 N-methoxysuccinyl-Ala-Ala-Pro-Val-4-nitroanilide pH 8.5, 37°C Dichelobacter nodosus
1.73
-
 N-methoxysuccinyl-Ala-Ala-Pro-Val-4-nitroanilide pH 8.5, 37°C, mutant D180G Dichelobacter nodosus
2.11
-
 N-methoxysuccinyl-Ala-Ala-Pro-Val-4-nitroanilide pH 8.5, 37°C, mutant D182G Dichelobacter nodosus
2.21
-
 N-methoxysuccinyl-Ala-Ala-Pro-Val-4-nitroanilide pH 8.5, 37°C, mutant D180G/D182G Dichelobacter nodosus
2.84
-
 N-methoxysuccinyl-Ala-Ala-Pro-Val-4-nitroanilide pH 8.5, 37°C Dichelobacter nodosus