Inhibitors | Comment | Organism | Structure |
---|---|---|---|
(dimethylamino)-1-naphthalenesulfonyl fluoride | 100% inhibition of enzyme activity with N-trans cinnamoyl imidazole as substrate, during prolonged exposure to organic solvents the active-site fluorescent label inhibitor adopts a different binding conformation | Bacillus licheniformis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus licheniformis | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | substrates bind in a less catalytically favorable conformation after the enzyme has been exposed to organic media for several hours | Bacillus licheniformis | ? | - |
? | |
N-trans-cinnamoyl imidazole + H2O | - |
Bacillus licheniformis | (E)-cinnamate + imidazole | - |
? | |
sec-phenethyl alcohol + vinyl butyrate | - |
Bacillus licheniformis | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
subtilisin C. | - |
Bacillus licheniformis |
subtilisin Carlsberg | - |
Bacillus licheniformis |
General Information | Comment | Organism |
---|---|---|
physiological function | when subtilisin C. is chemically modified with polyethylene glycol and inhibited with a dansyl fluorophore, and initially dissolved in two organic solvents (acetonitrile and 1,4-dioxane), the active site environment of the enzyme is similar to that in water. Prolonged exposure to the organic medium causes this environment to resemble that of the solvent in which the enzyme is dissolved | Bacillus licheniformis |