Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.4.21.62 extracted from

  • Pagan, M.; Sola, R.J.; Griebenow, K.
    On the role of protein structural dynamics in the catalytic activity and thermostability of serine protease subtilisin Carlsberg (2009), Biotechnol. Bioeng., 103, 77-84.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.4.21.62

Activating Compound

Activating Compound Comment Organism Structure
additional information glycosylation of subtilisin does not cause any significant tertiary structure changes synthetic construct

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0027
-
 Suc-Ala-Ala-Pro-Phe-4-nitroanilide 1 mol lactose bound per 1 mol subtilisin, in 10 mM potassium phosphate buffer, pH 7.8, at 25°C synthetic construct
0.0036
-
 Suc-Ala-Ala-Pro-Phe-4-nitroanilide 2.2 mol lactose bound per 1 mol subtilisin, in 10 mM potassium phosphate buffer, pH 7.8, at 25°C synthetic construct
0.0037
-
 Suc-Ala-Ala-Pro-Phe-4-nitroanilide 3.5 mol lactose bound per 1 mol subtilisin, in 10 mM potassium phosphate buffer, pH 7.8, at 25°C synthetic construct
0.0037
-
 Suc-Ala-Ala-Pro-Phe-4-nitroanilide subtilisin without bound lactose, in 10 mM potassium phosphate buffer, pH 7.8, at 25°C synthetic construct

Organism

Organism UniProt Comment Textmining
synthetic construct
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Suc-Ala-Ala-Pro-Phe-4-nitroanilide + H2O
-
 synthetic construct Suc-Ala-Ala-Pro-Phe + 4-nitroaniline
-
 ?

Synonyms

Synonyms Comment Organism
subtilisin Carlsberg
-
 synthetic construct

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
62 71 increasing the level of glycosylation causes a linearly dependent reduction in structural dynamics, which leads to an increase in thermostability: subtilisin without bound lactose: 64°C, 1 mol lactose bound per 1 mol subtilisin: 62°C, 2.2 mol lactose bound per 1 mol subtilisin: 69°C and 3.5 mol lactose bound per 1 mol subtilisin: 71°C synthetic construct

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
additional information
-
 additional information increasing the level of glycosylation causes a linearly dependent reduction in structural dynamics, which leads to a decrease in the catalytic turnover rate for both, the enzyme acylation and deacylation steps synthetic construct
90
-
 Suc-Ala-Ala-Pro-Phe-4-nitroanilide 3.5 mol lactose bound per 1 mol subtilisin, in 10 mM potassium phosphate buffer, pH 7.8, at 25°C synthetic construct
132
-
 Suc-Ala-Ala-Pro-Phe-4-nitroanilide 2.2 mol lactose bound per 1 mol subtilisin, in 10 mM potassium phosphate buffer, pH 7.8, at 25°C synthetic construct
166
-
 Suc-Ala-Ala-Pro-Phe-4-nitroanilide 1 mol lactose bound per 1 mol subtilisin, in 10 mM potassium phosphate buffer, pH 7.8, at 25°C synthetic construct
200
-
 Suc-Ala-Ala-Pro-Phe-4-nitroanilide subtilisin without bound lactose, in 10 mM potassium phosphate buffer, pH 7.8, at 25°C synthetic construct