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Literature summary for 3.4.21.62 extracted from

  • Wong, W.; Kennan, R.M.; Rosado, C.J.; Rood, J.I.; Whisstock, J.C.; Porter, C.J.
    Crystallization of the virulent and benign subtilisin-like proteases from the ovine footrot pathogen Dichelobacter nodosus (2010), Acta Crystallogr. Sect. F, 66, 289-293.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.4.21.62

Cloned(Commentary)

Cloned (Comment) Organism
vector pET22b-AprB2 encoded residues 1-474 of the AprB2 precursor with a C-terminal His6 transformed into Escherichia coli DH5alpha. Expression construct transformed into Escherichia coli strain Rosetta-Gami(DE3)pLysS Dichelobacter nodosus
vector pET22b-AprV2 encoded residues 1-474 of the AprV2 precursor with a C-terminal His6 tag transformed into Escherichia coli strain Rosetta-Gami(DE3)pLysS Dichelobacter nodosus
vector pET22b-BprB encoded residues 1-476 of BprB with a C-terminal His6 tag transformed into Escherichia coli strain Rosetta-Gami(DE3)pLysS Dichelobacter nodosus
vector pET22b-BprV encoded residues 1-476 of BprV with a C-terminal His6 tag and transformed into Escherichia coli strain Rosetta-Gami(DE3)pLysS Dichelobacter nodosus

Crystallization (Commentary)

Crystallization (Comment) Organism
by the hanging-drop vapour-diffusion method. Crystals of AprB2 diffract to 1.7 A resolution. The crystals belong to space group P1, with unit-cell parameters a = 42.7, b = 45.8, c = 45.7 A , alpha = 98.4, beta = 114.0, gamma = 114.6. The crystals contain one molecule in the asymmetric unit, with a solvent content of 36% Dichelobacter nodosus
by the hanging-drop vapour-diffusion method. Crystals of AprV2 diffract to 2.0 A resolution. The crystals belong to space group P1, with unit-cell parameters a = 43.1, b = 46.0, c = 47.2 A , alpha = 97.8, beta = 115.2, gamma = 115.2. The crystals contain one molecule in the asymmetric unit, with a solvent content of ca. 36% Dichelobacter nodosus
by the hanging-drop vapour-diffusion method. Crystals of BprB diffract to 1.8 A resolution. The crystals belong to space group P21, with unit-cell parameters a = 38.5, b = 90.5, c = 44.1 A , beta = 109.9. The crystals contain one molecule in the asymmetric unit, with a solvent content of 36% Dichelobacter nodosus
by the hanging-drop vapour-diffusion method. Crystals of BprV diffract to 2.0 A resolution. The crystals belong to space group P21, with unit-cell parameters a = 38.5, b = 89.6, c = 47.7 A , beta = 113.6. The crystals contain one molecule in the asymmetric unit, with a solvent content of 40% Dichelobacter nodosus

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ required for catalysis Dichelobacter nodosus
Ca2+ is required for catalysis Dichelobacter nodosus

Organism

Organism UniProt Comment Textmining
Dichelobacter nodosus
-
-
-
Dichelobacter nodosus A5EVD0 BprV
-
Dichelobacter nodosus A5EXI3 AprV2
-
Dichelobacter nodosus Q46547 BprB
-
Dichelobacter nodosus C305 Q46547 BprB
-
Dichelobacter nodosus VCS1703A A5EVD0 BprV
-
Dichelobacter nodosus VCS1703A A5EXI3 AprV2
-

Purification (Commentary)

Purification (Comment) Organism
by affinity chromatography Dichelobacter nodosus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
N-methoxysuccinyl-Ala-Ala-Pro-Val-p-nitroanilide + H2O
-
 Dichelobacter nodosus N-methoxysuccinyl-Ala-Ala-Pro-Val + p-nitroaniline
-
 ?
N-methoxysuccinyl-Ala-Ala-Pro-Val-p-nitroanilide + H2O
-
 Dichelobacter nodosus C305 N-methoxysuccinyl-Ala-Ala-Pro-Val + p-nitroaniline
-
 ?
N-methoxysuccinyl-Ala-Ala-Pro-Val-p-nitroanilide + H2O
-
 Dichelobacter nodosus VCS1703A N-methoxysuccinyl-Ala-Ala-Pro-Val + p-nitroaniline
-
 ?

Synonyms

Synonyms Comment Organism
subtilisin-like protease
-
 Dichelobacter nodosus

General Information

General Information Comment Organism
physiological function strains causing benign footrot secrete AprB2 Dichelobacter nodosus
physiological function strains causing benign footrot secrete subtilisin-like protease BprB Dichelobacter nodosus
physiological function strains causing virulent footrot secrete the subtilisin-like protease AprV2 Dichelobacter nodosus
physiological function strains causing virulent footrot secrete the subtilisin-like protease BprV Dichelobacter nodosus