Literature summary for 3.4.21.62 extracted from
Srimathi, S.; Jayaraman, G.; Narayanan, P.R.
Improved thermodynamic stability of subtilisin Carlsberg by covalent modification (2006), Enzyme Microb. Technol., 39, 301-307.
No PubMed abstract available
Activating Compound
Activating Compound |
Comment |
Organism |
Structure |
OSP400 |
in the presence of 10 mM calcium the half-life of the enzyme at 60°C increases by 6.06fold. Among modifiers used OSP400 is most effective in stabilizing the enzyme. Thermally induced unfolding is delayed |
Bacillus licheniformis |
|
OSP70 |
in the presence of 10 mM calcium the half-life of the enzyme at 60°C increases by 5.20fold. Thermally induced unfolding is delayed |
Bacillus licheniformis |
|
polyglutaraldehyde |
in the presence of 10 mM calcium the half-life of the enzyme at 60°C increases by 2.92fold. Thermally induced unfolding is delayed |
Bacillus licheniformis |
|
Application
Application |
Comment |
Organism |
industry |
improving the kinetic, thermal and thermodynamic stability of subtilisin Carlsberg by means of simple, inexpensive but effective covalent coupling to oxidized sucrose polymers of varying sizes as well as polyglutaraldehyde. Stability is comparable to the most stabilized subtilisin variants obtained by site-directed mutagenesis |
Bacillus licheniformis |
Metals/Ions
Metals/Ions |
Comment |
Organism |
Structure |
Ca2+ |
in the presence of calcium the half-life at 55°C and 60°C are 3.6fold and 3.48fold higher for the native enzyme compared to that in the absence of added calcium. In the presence of 10 mM calcium the half-life of the enzyme at 60°C increases by 6.06fold, 5.20fold and 2.92fold when coupled with oxidized sucrose polymers OSP400, OSP70 and polyglutaraldehyde, respectively |
Bacillus licheniformis |
|
Molecular Weight [Da]
Molecular Weight [Da] |
Molecular Weight Maximum [Da] |
Comment |
Organism |
27000 |
- |
1 * 27000, SDS-PAGE |
Bacillus licheniformis |
Organism
Organism |
UniProt |
Comment |
Textmining |
Bacillus licheniformis |
- |
- |
- |
Purification (Commentary)
Purification (Comment) |
Organism |
by ammonium sulfate precipitation followed by gel filtration |
Bacillus licheniformis |
Substrates and Products (Substrate)
Substrates |
Comment Substrates |
Organism |
Products |
Comment (Products) |
Rev. |
Reac. |
casein + H2O |
- |
Bacillus licheniformis |
? |
- |
? |
|
Subunits
Subunits |
Comment |
Organism |
monomer |
1 * 27000, SDS-PAGE |
Bacillus licheniformis |
Synonyms
Synonyms |
Comment |
Organism |
subtilisin Carlsberg |
- |
Bacillus licheniformis |
Temperature Optimum [°C]
Temperature Optimum [°C] |
Temperature Optimum Maximum [°C] |
Comment |
Organism |
67 |
- |
native subtilisin and its modified counterparts |
Bacillus licheniformis |
Temperature Range [°C]
Temperature Minimum [°C] |
Temperature Maximum [°C] |
Comment |
Organism |
55 |
76.6 |
- |
Bacillus licheniformis |
pH Optimum
pH Optimum Minimum |
pH Optimum Maximum |
Comment |
Organism |
10.5 |
- |
native subtilisin and its modified counterparts |
Bacillus licheniformis |
pH Range
pH Minimum |
pH Maximum |
Comment |
Organism |
3.5 |
11 |
native subtilisin and its modified counterparts |
Bacillus licheniformis |