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Literature summary for 3.4.21.62 extracted from

  • Srimathi, S.; Jayaraman, G.; Narayanan, P.R.
    Improved thermodynamic stability of subtilisin Carlsberg by covalent modification (2006), Enzyme Microb. Technol., 39, 301-307.
No PubMed abstract available

Activating Compound

Activating Compound Comment Organism Structure
OSP400 in the presence of 10 mM calcium the half-life of the enzyme at 60°C increases by 6.06fold. Among modifiers used OSP400 is most effective in stabilizing the enzyme. Thermally induced unfolding is delayed Bacillus licheniformis
OSP70 in the presence of 10 mM calcium the half-life of the enzyme at 60°C increases by 5.20fold. Thermally induced unfolding is delayed Bacillus licheniformis
polyglutaraldehyde in the presence of 10 mM calcium the half-life of the enzyme at 60°C increases by 2.92fold. Thermally induced unfolding is delayed Bacillus licheniformis

Application

Application Comment Organism
industry improving the kinetic, thermal and thermodynamic stability of subtilisin Carlsberg by means of simple, inexpensive but effective covalent coupling to oxidized sucrose polymers of varying sizes as well as polyglutaraldehyde. Stability is comparable to the most stabilized subtilisin variants obtained by site-directed mutagenesis Bacillus licheniformis

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ in the presence of calcium the half-life at 55°C and 60°C are 3.6fold and 3.48fold higher for the native enzyme compared to that in the absence of added calcium. In the presence of 10 mM calcium the half-life of the enzyme at 60°C increases by 6.06fold, 5.20fold and 2.92fold when coupled with oxidized sucrose polymers OSP400, OSP70 and polyglutaraldehyde, respectively Bacillus licheniformis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
27000
-
1 * 27000, SDS-PAGE Bacillus licheniformis

Organism

Organism UniProt Comment Textmining
Bacillus licheniformis
-
-
-

Purification (Commentary)

Purification (Comment) Organism
by ammonium sulfate precipitation followed by gel filtration Bacillus licheniformis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
casein + H2O
-
Bacillus licheniformis ?
-
?

Subunits

Subunits Comment Organism
monomer 1 * 27000, SDS-PAGE Bacillus licheniformis

Synonyms

Synonyms Comment Organism
subtilisin Carlsberg
-
Bacillus licheniformis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
67
-
native subtilisin and its modified counterparts Bacillus licheniformis

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
55 76.6
-
Bacillus licheniformis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
10.5
-
native subtilisin and its modified counterparts Bacillus licheniformis

pH Range

pH Minimum pH Maximum Comment Organism
3.5 11 native subtilisin and its modified counterparts Bacillus licheniformis