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Literature summary for 3.4.21.62 extracted from

  • Shah, S.; Gupta, M.N.
    Obtaining high transesterification activity for subtilisin in ionic liquids (2007), Biochim. Biophys. Acta, 1770, 94-98.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
additional information three phase partitioning reveals 13fold increase in the initial transesterfication rate Bacillus licheniformis
n-Propanol subtilisin precipitated and rinsed with propanol yields higher transesterfication activity. About 10000times increase in initial rates in 1-butyl-3-methylimidazolium hexafluorophosphate over what is obtained with pH tuned lyophilized powders Bacillus licheniformis
poly(ethyleneglycol) presence of poly(ethyleneglycol) combined with trehalose during lyphilization does help in obtaining a more active enzyme preparation for catalysis in ionic liquids Bacillus licheniformis

Application

Application Comment Organism
additional information biocatalyst preparations which are capable of yielding higher/adequate transesterifaction activity of subtilisin in ionic liquids Bacillus licheniformis

Organism

Organism UniProt Comment Textmining
Bacillus licheniformis
-
-
-

Storage Stability

Storage Stability Organism
-20°C Bacillus licheniformis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
N-acetyl-L-phenylalanine ethyl ester
-
Bacillus licheniformis ?
-
?

Synonyms

Synonyms Comment Organism
subtilisin Carlsberg
-
Bacillus licheniformis