Activating Compound | Comment | Organism | Structure |
---|---|---|---|
additional information | prodomain mutant proR9 accelerates subtilisin folding. Subtilisin folding with the wild-type prodomain is slow. The wild-type prodomain binds 100times slower than proR9 | Bacillus amyloliquefaciens |
Application | Comment | Organism |
---|---|---|
additional information | slow folding of subtilisin results from the formation of an unstable and topologically challenged intermediate and the proline-limited isomerization of the intermediate to the native state | Bacillus amyloliquefaciens |
Cloned (Comment) | Organism |
---|---|
inactive mutants of subtilisin BPN expressed in Bacillus subtilis. Expression of prodomain mutant proR9 in Escherichia coli strain BL21(DE3) | Bacillus amyloliquefaciens |
Protein Variants | Comment | Organism |
---|---|---|
D32N | does not fold at all in the presence of proR9 | Bacillus amyloliquefaciens |
N155L | folding kinetics very similar to that of S221A | Bacillus amyloliquefaciens |
P168G | weaker binding of proR9 | Bacillus amyloliquefaciens |
S221A | a delta75-83 mutant. Shows slow folding reaction. Is unstable at low metal concentration, unfolding at a rate of 0.6 h-1. If denatured, it is refolded with an excess of proR9 | Bacillus amyloliquefaciens |
S221C | folding kinetics very similar to that of S221A | Bacillus amyloliquefaciens |
T22C/S87C/S221C | if denatured, it is refolded with an excess of proR9. About 75% is rapidly bound to proR9. 25% undergoes a slow step prior to binding | Bacillus amyloliquefaciens |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | metal ion sites are not formed until late in folding and do not influence the kinetics of folding to the intermediate complex | Bacillus amyloliquefaciens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus amyloliquefaciens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Bacillus amyloliquefaciens |
Storage Stability | Organism |
---|---|
-20°C | Bacillus amyloliquefaciens |
Synonyms | Comment | Organism |
---|---|---|
subtilisin BPN | - |
Bacillus amyloliquefaciens |