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Literature summary for 3.4.21.62 extracted from

  • Tindbaek, N.; Svendsen, A.; Oestergaard, P.R.; Draborg, H.
    Engineering a substrate-specific cold-adapted subtilisin (2004), Protein Eng. Des. Sel., 17, 149-156.
    View publication on PubMed
Search for more literature for 3.4.21.62

Protein Variants

Protein Variants Comment Organism
V104S/L124M/P129S/S130G/P131E/A133S/T134L/L135I mutant containing the highly flexible region of psychrophilic enzyme TA39 subtilisin. Mutant shows the same temperature optimum and pH-profile as wild-type, but higher specificity for substrate succinyl-L-Ala-L-Ala-L-Pro-L-Phe-4-nitroanilide and broader substrate specificity. Mutant has a decreased thermostability, but increased activity at low temperature Lederbergia lentus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.039
-
 L-Ala-L-Ala-L-Val-L-Ala wild-type, pH 9.0, 24°C Lederbergia lentus
0.053
-
 L-Ala-L-Ala-L-Val-L-Ala V104S/L124M/P129S/S130G/P131E/A133S/T134L/L135I, pH 9.0, 24°C Lederbergia lentus
0.223
-
 L-Phe-L-Ala-L-Ala-L-Phe wild-type, pH 9.0, 24°C Lederbergia lentus
0.24
-
 L-Phe-L-Ala-L-Ala-L-Phe V104S/L124M/P129S/S130G/P131E/A133S/T134L/L135I, pH 9.0, 24°C Lederbergia lentus
0.62
-
 L-Tyr-L-Val-L-Ala-L-Asp wild-type, pH 9.0, 24°C Lederbergia lentus
0.75
-
 L-Tyr-L-Val-L-Ala-L-Asp V104S/L124M/P129S/S130G/P131E/A133S/T134L/L135I, pH 9.0, 24°C Lederbergia lentus
0.98
-
 L-Ala-L-Ala-L-Pro-L-Ala V104S/L124M/P129S/S130G/P131E/A133S/T134L/L135I, pH 9.0, 24°C Lederbergia lentus
1.15
-
 L-Ala-L-Ala-L-Pro-L-Phe wild-type, pH 9.0, 24°C Lederbergia lentus
1.93
-
 L-Ala-L-Ala-L-Pro-L-Phe V104S/L124M/P129S/S130G/P131E/A133S/T134L/L135I, pH 9.0, 24°C Lederbergia lentus
1.99
-
 L-Ala-L-Ala-L-Pro-L-Ala wild-type, pH 9.0, 24°C Lederbergia lentus

Organism

Organism UniProt Comment Textmining
Lederbergia lentus
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-Ala-L-Ala-L-Pro-L-Ala + H2O
-
 Lederbergia lentus ?
-
 ?
L-Ala-L-Ala-L-Pro-L-Phe + H2O
-
 Lederbergia lentus ?
-
 ?
L-Ala-L-Ala-L-Val-L-Ala + H2O
-
 Lederbergia lentus ?
-
 ?
L-Phe-L-Ala-L-Ala-L-Phe + H2O
-
 Lederbergia lentus ?
-
 ?
L-Tyr-L-Val-L-Ala-L-Asp + H2O
-
 Lederbergia lentus ?
-
 ?
succinyl-L-Ala-L-Ala-L-Pro-L-Phe-4-nitroanilide + H2O
-
 Lederbergia lentus ?
-
 ?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4
-
 L-Tyr-L-Val-L-Ala-L-Asp V104S/L124M/P129S/S130G/P131E/A133S/T134L/L135I, pH 9.0, 24°C Lederbergia lentus
10
-
 L-Ala-L-Ala-L-Val-L-Ala V104S/L124M/P129S/S130G/P131E/A133S/T134L/L135I, pH 9.0, 24°C Lederbergia lentus
10
-
 L-Tyr-L-Val-L-Ala-L-Asp wild-type, pH 9.0, 24°C Lederbergia lentus
12
-
 L-Ala-L-Ala-L-Val-L-Ala wild-type, pH 9.0, 24°C Lederbergia lentus
31
-
 L-Ala-L-Ala-L-Pro-L-Ala V104S/L124M/P129S/S130G/P131E/A133S/T134L/L135I, pH 9.0, 24°C Lederbergia lentus
47
-
 L-Ala-L-Ala-L-Pro-L-Ala wild-type, pH 9.0, 24°C Lederbergia lentus
90
-
 L-Phe-L-Ala-L-Ala-L-Phe wild-type, pH 9.0, 24°C Lederbergia lentus
185
-
 L-Ala-L-Ala-L-Pro-L-Phe wild-type, pH 9.0, 24°C Lederbergia lentus
189
-
 L-Phe-L-Ala-L-Ala-L-Phe V104S/L124M/P129S/S130G/P131E/A133S/T134L/L135I, pH 9.0, 24°C Lederbergia lentus
403
-
 L-Ala-L-Ala-L-Pro-L-Phe V104S/L124M/P129S/S130G/P131E/A133S/T134L/L135I, pH 9.0, 24°C Lederbergia lentus