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Literature summary for 3.4.21.62 extracted from

  • Strausberg, S.L.; Ruan, B.; Fisher, K.E.; Alexander, P.A.; Bryan, P.N.
    Directed coevolution of stability and catalytic activity in calcium-free subtilisin (2005), Biochemistry, 44, 3272-3279.
    View publication on PubMed
Search for more literature for 3.4.21.62

Protein Variants

Protein Variants Comment Organism
additional information sequential randomization of 12 amino acid positions in calcium-free enzyme. Mutations increase the half-life of enzyme at elevated temperature by 15000fold and partially increase ratio of kcat/KM-value Bacillus amyloliquefaciens
Y217L commercial version of enzyme, i.e. SBT* Bacillus amyloliquefaciens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.33
-
 succinyl-L-Asp-L-Val-L-Arg-L-Ala-L-Phe-7-amido-4-methylcoumarin mutant Y217L, pH 8.3, 25°C Bacillus amyloliquefaciens
0.36
-
 succinyl-L-Ala-L-Ala-L-Phe-7-amido-4-methylcoumarin mutant Y217L, pH 8.3, 25°C Bacillus amyloliquefaciens
0.45
-
 succinyl-L-Ala-L-Ala-L-Pro-L-Phe-4-nitroanilide mutant Y217L, pH 8.3, 25°C Bacillus amyloliquefaciens

Organism

Organism UniProt Comment Textmining
Bacillus amyloliquefaciens
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
succinyl-L-Ala-L-Ala-L-Phe-7-amido-4-methylcoumarin + H2O
-
 Bacillus amyloliquefaciens succinyl-L-Ala-L-Ala-L-Phe + 7-amino-4-methylcoumarin
-
 ?
succinyl-L-Ala-L-Ala-L-Pro-L-Phe-4-nitroanilide + H2O
-
 Bacillus amyloliquefaciens succinyl-L-Ala-L-Ala-L-Pro-L-Phe + 4-nitroaniline
-
 ?
succinyl-L-Asp-L-Val-L-Arg-L-Ala-L-Phe-7-amido-4-methylcoumarin + H2O
-
 Bacillus amyloliquefaciens succinyl-L-Asp-L-Val-L-Arg-L-Ala-L-Phe + 7-amino-4-methylcoumarin
-
 ?

Synonyms

Synonyms Comment Organism
subtilisin BPN’
-
 Bacillus amyloliquefaciens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
6.4
-
 succinyl-L-Ala-L-Ala-L-Phe-7-amido-4-methylcoumarin mutant Y217L, pH 8.3, 25°C Bacillus amyloliquefaciens
68
-
 succinyl-L-Asp-L-Val-L-Arg-L-Ala-L-Phe-7-amido-4-methylcoumarin mutant Y217L, pH 8.3, 25°C Bacillus amyloliquefaciens
210
-
 succinyl-L-Ala-L-Ala-L-Pro-L-Phe-4-nitroanilide mutant Y217L, pH 8.3, 25°C Bacillus amyloliquefaciens