Any feedback?
Literature summary for 3.4.21.62 extracted from
- Engineering subtilisin into a fluoride-triggered processing protease useful for one-step protein purification (2004), Biochemistry, 43, 14539-14546.
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| additional information | introduction of about 20 site-directed mutations to obtain a variant with increased sequence selectivity, a predomain that can direct cleavage to the junction of any protein fused to it, and a modified active site to kinetically isolate binding and cleavage reactions. Identification of specific anions that trigger the processing reaction so that column-immobilized mutant enzyme can be used as both the affinity ligand and the processing protease for one-step purification methods | Bacillus amyloliquefaciens |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bacillus amyloliquefaciens | - |
recombinant protein | - |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
