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Literature summary for 3.4.21.62 extracted from
- Purification and characterization of subtilisin DJ-4 secreted by Bacillus sp. strain DJ-4 screened from Doen-Jang (2000), Biosci. Biotechnol. Biochem., 64, 1722-1725.
Application
| Application | Comment | Organism |
|---|---|---|
| medicine | fibrinolytic activiy of strain DJ-4, develop the enzyme for its use as a thrombolytic agent | Bacillus sp. (in: Bacteria) |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Cu2+ | strain DJ-4, strong inhibition | Bacillus sp. (in: Bacteria) |  |
| additional information | strain DJ-4, no inhibition by EDTA or leupeptin | Bacillus sp. (in: Bacteria) | |
| phenylmethylsulfonyl fluoride | strain DJ-4 | Bacillus sp. (in: Bacteria) | |
| Zn2+ | strain DJ-4, strong inhibition | Bacillus sp. (in: Bacteria) | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| extracellular | - |
Bacillus sp. (in: Bacteria) | - |
- |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| additional information | strain DJ-4, not significantly affected by Ca2+, Co2+, Mg2+, or Mn2+ | Bacillus sp. (in: Bacteria) |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 29000 | - |
strain DJ-4, SDS-PAGE | Bacillus sp. (in: Bacteria) |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bacillus sp. (in: Bacteria) | - |
isolated from Korean fermented soybean food, Doen-Jang | - |
| Bacillus sp. (in: Bacteria) DJ-4 | - |
isolated from Korean fermented soybean food, Doen-Jang | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| strain DJ-4 | Bacillus sp. (in: Bacteria) |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
strain DJ-4, specific activity 0.98 | Bacillus sp. (in: Bacteria) |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| Fibrin + H2O | strain DJ-4 | Bacillus sp. (in: Bacteria) | ? | - |
? | |
| Fibrin + H2O | strain DJ-4 | Bacillus sp. (in: Bacteria) DJ-4 | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 29000, strain DJ-4, SDS-PAGE | Bacillus sp. (in: Bacteria) |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| subtilisin DJ-4 | strain DJ-4 | Bacillus sp. (in: Bacteria) |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
strain DJ-4 | Bacillus sp. (in: Bacteria) |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
strain DJ-4, 80% of the enzyme's activity remains after incubation for 1 h | Bacillus sp. (in: Bacteria) |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 10 | - |
strain DJ-4 | Bacillus sp. (in: Bacteria) |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 4 | 11 | strain DJ-4 | Bacillus sp. (in: Bacteria) |
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