Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.4.21.62 extracted from

  • Alexander, P.A.; Ruan, B.; Strausberg, S.L.; Bryan, P.N.
    Stabilizing mutations and calcium dependent stability of subtilisin (2001), Biochemistry, 40, 10640-10644.
    View publication on PubMed
Search for more literature for 3.4.21.62

Application

Application Comment Organism
analysis popular system for protein engineering studies Bacillus amyloliquefaciens
synthesis subtilisin BPN' is an important industrial enzyme Bacillus amyloliquefaciens

Cloned(Commentary)

Cloned (Comment) Organism
subtilisin BPN' cloned, sequenced and expressed at high levels from its natural promoter sequences in Bacillus subtilis Bacillus amyloliquefaciens

Protein Variants

Protein Variants Comment Organism
G166S site-directed mutagenesis, BPN' Bacillus amyloliquefaciens
G169A stable only in presence of excess Ca2+ Bacillus amyloliquefaciens
L126I site-directed mutagenesis, BPN', stable only in presence of excess Ca2+ Bacillus amyloliquefaciens
M50F site-directed mutagenesis, BPN',stabilized in EDTA, but destabilized slightly in CaCl2 Bacillus amyloliquefaciens
N218S site-directed mutagenesis, BPN', stabilized significantly, either with or without Ca2+ Bacillus amyloliquefaciens
Q206Cox site-directed mutagenesis, BPN' Bacillus amyloliquefaciens
Q271E site-directed mutagenesis, BPN', stabilized significantly, either with or without Ca2+ Bacillus amyloliquefaciens
S188P site-directed mutagenesis, BPN', stable only in presence of excess Ca2+ Bacillus amyloliquefaciens
T254A site-directed mutagenesis, BPN', stable only in presence of excess Ca2+ Bacillus amyloliquefaciens
Y217K site-directed mutagenesis, BPN', stabilized significantly, either with or without Ca2+ Bacillus amyloliquefaciens

General Stability

General Stability Organism
stability is a property of subtilisin which has proven particularly amenable to enhancement via random mutagenesis Bacillus amyloliquefaciens

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ stabilizing mutations are usually calcium-dependent in their stabilizing effect Bacillus amyloliquefaciens

Organism

Organism UniProt Comment Textmining
Bacillus amyloliquefaciens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
subtilisin BPN' and recombinant variants Bacillus amyloliquefaciens

Synonyms

Synonyms Comment Organism
BPN'
-
 Bacillus amyloliquefaciens