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Literature summary for 3.4.21.62 extracted from

  • Jang, J.S.; Kang, D.O.; Park, K.S.; Byun, S.M.
    Purification and characterization of recombinant Bacillus stearothermophilus subtilisin J (1993), Korean Biochem. J., 26, 595-601.
No PubMed abstract available

Cloned(Commentary)

Cloned (Comment) Organism
Bacillus stearothermophilus NCIMB10278 gene expressed in Bacillus subtilis DB104/pZS101 Geobacillus stearothermophilus

General Stability

General Stability Organism
Ca2+ stabilizes the enzyme upon heat treatment Geobacillus stearothermophilus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
27500
-
Bacillus stearothermophilus NCIMB10278 gene expressed in Bacillus subtilis DB104/pZS101 Geobacillus stearothermophilus

Organism

Organism UniProt Comment Textmining
Geobacillus stearothermophilus
-
subtilisin J, Bacillus stearothermophilus NCIMB10278 gene expressed in Bacillus subtilis DB104/pZS101
-

Purification (Commentary)

Purification (Comment) Organism
NCIMB10278, gene expressed in Bacillus subtilis DB104/pZS101 Geobacillus stearothermophilus

Source Tissue

Source Tissue Comment Organism Textmining
culture medium
-
Geobacillus stearothermophilus
-

Synonyms

Synonyms Comment Organism
Subtilisn J
-
Geobacillus stearothermophilus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60
-
-
Geobacillus stearothermophilus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
additional information
-
Ca2+ stabilizes the enzyme upon heat treatment Geobacillus stearothermophilus
60
-
30 min, presence of 2 mM calcium, about 50% loss of activity Geobacillus stearothermophilus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9
-
-
Geobacillus stearothermophilus