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Literature summary for 3.4.21.62 extracted from

  • Yamagata, Y.; Isshiki, K.; Ichishima, E.
    Subtilisin Sendai from alkalophilic Bacillus sp.: molecular and enzymatic properties of the enzyme and molecular cloning and characterization of the gene, aprS (1995), Enzyme Microb. Technol., 17, 653-663.
    View publication on PubMed
Search for more literature for 3.4.21.62

Cloned(Commentary)

Cloned (Comment) Organism
-
 Bacillus sp. (in: Bacteria)

Inhibitors

Inhibitors Comment Organism Structure
antipain
-
 Bacillus sp. (in: Bacteria)
chymostatin
-
 Bacillus sp. (in: Bacteria)
Elastatinol weak Bacillus sp. (in: Bacteria)
PMSF
-
 Bacillus sp. (in: Bacteria)
tosyl-Phe chloromethyl ketone
-
 Bacillus sp. (in: Bacteria)

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information
-
 Bacillus sp. (in: Bacteria)
0.13
-
 succinyl-Ala-Ala-Pro-Phe 4-nitroanilide succinyl-Leu-Leu-Val-Tyr-4-methylcoumaryl 7-amide, subtilisin Sendai Bacillus sp. (in: Bacteria)

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
 Bacillus sp. (in: Bacteria)
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
27230
-
 subtilisin Sendai, amino acid sequence Bacillus sp. (in: Bacteria)
28000
-
 x * 28000, Bacillus sp., SDS-PAGE Bacillus sp. (in: Bacteria)

Organism

Organism UniProt Comment Textmining
Bacillus sp. (in: Bacteria)
-
 subtilisin Sendai
-
Bacillus sp. (in: Bacteria) G-825-6
-
 subtilisin Sendai
-

Purification (Commentary)

Purification (Comment) Organism
-
 Bacillus sp. (in: Bacteria)

Source Tissue

Source Tissue Comment Organism Textmining
culture medium
-
 Bacillus sp. (in: Bacteria)
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Oxidized insulin B-chain + H2O enzyme primarily hydrolyzes Leu15-Tyr16 bond and secondarily Gln4-His5, Ser9-His10, Phe24-Phe25 and Lys29-Ala30 Bacillus sp. (in: Bacteria) ?
-
 ?
Oxidized insulin B-chain + H2O enzyme primarily hydrolyzes Leu15-Tyr16 bond and secondarily Gln4-His5, Ser9-His10, Phe24-Phe25 and Lys29-Ala30 Bacillus sp. (in: Bacteria) G-825-6 ?
-
 ?
Succinyl-Leu-Leu-Val-Tyr 4-methylcoumarin 7-amide + H2O
-
 Bacillus sp. (in: Bacteria) ?
-
 ?
Succinyl-Leu-Leu-Val-Tyr 4-methylcoumarin 7-amide + H2O
-
 Bacillus sp. (in: Bacteria) G-825-6 ?
-
 ?

Subunits

Subunits Comment Organism
? x * 28000, Bacillus sp., SDS-PAGE Bacillus sp. (in: Bacteria)

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
 succinyl-Leu-Leu-Val-Tyr-4-methylcoumaryl 7-amide Bacillus sp. (in: Bacteria)

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
65
-
 pH 10.0, 10 min, stable up to Bacillus sp. (in: Bacteria)
70
-
 pH 10.0, 10 min, complete loss of activity Bacillus sp. (in: Bacteria)

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
additional information
-
 additional information
-
 Bacillus sp. (in: Bacteria)
29
-
 succinyl-Leu-Leu-Val-Tyr-4-methylcoumaryl 7-amide subtilisin Sendai Bacillus sp. (in: Bacteria)

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
10
-
 succinyl-Leu-Leu-Val-Tyr 4-methylcoumarin 7-amide Bacillus sp. (in: Bacteria)