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Literature summary for 3.4.21.62 extracted from

  • Thomas, P.G.; Russell, A.J.; Fersht, A.R.
    Tailoring the pH dependence of enzyme catalysis using protein engineering (1985), Nature, 318, 375-376.
No PubMed abstract available

Cloned(Commentary)

Cloned (Comment) Organism
-
Bacillus sp. (in: Bacteria)
-
Bacillus amyloliquefaciens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.13
-
succinyl-Ala-Ala-Pro-Phe 4-nitroanilide
-
Bacillus sp. (in: Bacteria)
0.13
-
succinyl-Ala-Ala-Pro-Phe 4-nitroanilide mutant subtilisin BPN' Asp to Ser99 Bacillus amyloliquefaciens
0.15
-
MeO-succinyl-Ala-Ala-Phe 4-nitroanilide
-
Bacillus sp. (in: Bacteria)
0.15
-
MeO-succinyl-Ala-Ala-Phe 4-nitroanilide succinyl-Ala-Ala-Pro-Phe 4-nitroanilide, subtilisin BPN' Bacillus amyloliquefaciens

Organism

Organism UniProt Comment Textmining
Bacillus amyloliquefaciens
-
wild-type and mutant (Asp to Ser99), the wild-type subtilisin BPN' is expressed and secreted by Bacillus subtilis DB104
-
Bacillus sp. (in: Bacteria)
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
MeO-succinyl-Ala-Ala-Phe 4-nitroanilide + H2O
-
Bacillus sp. (in: Bacteria) ?
-
?
MeO-succinyl-Ala-Ala-Phe 4-nitroanilide + H2O
-
Bacillus amyloliquefaciens ?
-
?
succinyl-Ala-Ala-Pro-Phe 4-nitroanilide + H2O
-
Bacillus sp. (in: Bacteria) succinyl-Ala-Ala-Pro-Phe + 4-nitroaniline
-
?
succinyl-Ala-Ala-Pro-Phe 4-nitroanilide + H2O
-
Bacillus amyloliquefaciens succinyl-Ala-Ala-Pro-Phe + 4-nitroaniline
-
?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
45
-
succinyl-Ala-Ala-Pro-Phe 4-nitroanilide mutant subtilisin BPN' Asp99Ser Bacillus amyloliquefaciens
57
-
MeO-succinyl-Ala-Ala-Phe 4-nitroanilide subtilisin Carlsberg Bacillus sp. (in: Bacteria)
57
-
MeO-succinyl-Ala-Ala-Phe 4-nitroanilide subtilisin Carlsberg Bacillus amyloliquefaciens
57
-
succinyl-Ala-Ala-Pro-Phe 4-nitroanilide subtilisin BPN' Bacillus sp. (in: Bacteria)
57
-
succinyl-Ala-Ala-Pro-Phe 4-nitroanilide subtilisin BPN' Bacillus amyloliquefaciens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
additional information
-
-
Bacillus sp. (in: Bacteria)
additional information
-
mutant subtilisin Asp to Ser99, change of just one surface charge which is 14-15 A from the active site of subtilisin has a significant effect on the pH-dependence Bacillus amyloliquefaciens