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Literature summary for 3.4.21.62 extracted from
- Proteases of enhanced stability: characterization of a thermostable variant of subtilisin (1986), Proteins Struct. Funct. Genet., 1, 326-334.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| subtilisin 7150, mutagenized subtilisin with enhanced thermal stability | Bacillus amyloliquefaciens |
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
| wild-type and thermostable mutant 7150 subtilisin at 1.8 A resolution | Bacillus amyloliquefaciens |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bacillus amyloliquefaciens | - |
wild-type and subtilisin 7150 (mutagenized subtilisin with enhanced thermal stability) | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Bacillus amyloliquefaciens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| succinyl-Ala-Ala-Pro-Phe 4-nitroanilide + H2O | - |
Bacillus amyloliquefaciens | succinyl-Ala-Ala-Pro-Phe + 4-nitroaniline | - |
? |  |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 65 | - |
pH 8.0, 10 mM CaCl2, half-life of wild-type enzyme: 59 min, half-life of mutant 7150 enzyme: 223 min | Bacillus amyloliquefaciens |
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