Literature summary for 3.4.21.60 extracted from

Senis, Y.A.; Kim, P.Y.; Fuller, G.L.; Garcia, A.; Prabhakar, S.; Wilkinson, M.C.; Brittan, H.; Zitzmann, N.; Wait, R.; Warrell, D.A.; Watson, S.P.; Kamiguti, A.S.; Theakston, R.D.; Nesheim, M.E.; Laing, G.D.
Isolation and characterization of cotiaractivase, a novel low molecular weight prothrombin activator from the venom of Bothrops cotiara (2006), Biochim. Biophys. Acta, 1764, 863-871.

Search for more literature for 3.4.21.60

Inhibitors

Inhibitors	Comment	Organism	Structure
EDTA	5 mM, complete inhibition	Bothrops cotiara
additional information	not inhibitory: phenylmethyl sulfonylfluoride	Bothrops cotiara
S2238	noncompetitive	Bothrops cotiara

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.0127	-	prothrombin	22°C, pH 7.4, presence of S2238	Bothrops cotiara
0.0177	-	prothrombin	22°C, pH 7.4	Bothrops cotiara

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Ca2+	required	Bothrops cotiara

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
22931	-	x * 22931, mass spectroscopy, x * 23000, SDS-PAGE	Bothrops cotiara
23000	-	x * 22931, mass spectroscopy, x * 23000, SDS-PAGE	Bothrops cotiara

Organism

Organism	UniProt	Comment	Textmining
Bothrops cotiara	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
-	Bothrops cotiara

Source Tissue

Source Tissue	Comment	Organism	Textmining
venom	-	Bothrops cotiara	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
prothrombin + H2O	cleavage at positions Arg271-Thr272 and Arg320-Ile321	Bothrops cotiara	alpha-thrombin + ?	-	?

Subunits

Subunits	Comment	Organism
?	x * 22931, mass spectroscopy, x * 23000, SDS-PAGE	Bothrops cotiara

Synonyms

Synonyms	Comment	Organism
cotiaractivase	-	Bothrops cotiara

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
0.00015	-	prothrombin	22°C, pH 7.4, presence of S2238	Bothrops cotiara
0.00027	-	prothrombin	22°C, pH 7.4	Bothrops cotiara

Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.

Release 2023.1 (January 2023)