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Literature summary for 3.4.21.6 extracted from

  • Buddai, S.K.; Layzer, J.M.; Lu, G.; Rusconi, C.P.; Sullenger, B.A.; Monroe, D.M.; Krishnaswamy, S.
    An anticoagulant RNA aptamer that inhibits proteinase-cofactor interactions within prothrombinase (2010), J. Biol. Chem., 285, 5212-5223.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.4.21.6

Inhibitors

Inhibitors Comment Organism Structure
RNA11F7t a RNA aptamer, that is selected from a combinatorial library based on its ability to bind factor Xa, inhibits thrombin formation catalyzed by prothrombinase without obscuring the active site of Xa within the enzyme complex. Selective inhibition of protein substrate cleavage arises from the ability of the aptamer to bind to factor Xa and exclude interactions between the proteinase and cofactor within prothrombinase. Competition for enzyme complex assembly results from the binding of RNA11F7t to factor Xa with nanomolar affinity in a Ca2+-dependent interaction. RNA11F7t binds equivalently to the zymogen factor X as well as derivatives lacking gamma-carboxyglutamic acid residues, strong Ca2+-dependence of the binding ofRNA1F7t Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane interaction of factor Xa with factor Va on membranes to form prothrombinase Homo sapiens 16020
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Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
prothrombin + H2O Homo sapiens the interaction of factor Xa with factor Va on membranes to form prothrombinase profoundly increases the rate of the proteolytic conversion of prothrombin to thrombin thrombin + ?
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 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
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Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
prothrombin + H2O
-
 Homo sapiens thrombin + ?
-
 ?
prothrombin + H2O the interaction of factor Xa with factor Va on membranes to form prothrombinase profoundly increases the rate of the proteolytic conversion of prothrombin to thrombin Homo sapiens thrombin + ?
-
 ?

Synonyms

Synonyms Comment Organism
prothrombinase
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 Homo sapiens