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Literature summary for 3.4.21.6 extracted from

  • Srivastava, A.; Wang, J.; Majumder, R.; Rezaie, A.R.; Stenflo, J.; Esmon, C.T.; Lentz, B.R.
    Localization of phosphatidylserine binding sites to structural domains of factor Xa (2002), J. Biol. Chem., 277, 1855-1863.
    View publication on PubMed
Search for more literature for 3.4.21.6

Activating Compound

Activating Compound Comment Organism Structure
short chain phosphatidylserine i.e. C6PS, binding leads to 60fold increased proteolytic activity, location of binding sites and binding structure, partly Ca2+-dependent, kinetics with wild-type enzyme and mutant enzyme fragments Homo sapiens
short chain phosphatidylserine i.e. C6PS, binding leads to 60fold increased proteolytic activity, location of binding sites and binding structure, partly Ca2+-dependent, kinetics with wild-type enzyme and mutant enzyme fragments Bos taurus

Cloned(Commentary)

Cloned (Comment) Organism
expression of wild-type enzyme, enzyme lacking the Gla- and the EGF-like domains, of the isolated Gla-domain, the EGF-like domain, and a construct comprising both, expression in human 293 cells Homo sapiens
expression of wild-type enzyme, enzyme lacking the Gla- and the EGF-like domains, of the isolated Gla-domain, the EGF-like domain, and a construct comprising both, expression in human 293 cells Bos taurus

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
 Homo sapiens
-
-
extracellular
-
 Bos taurus
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ required for binding of the EGF-like domains at the catalytic C-terminus and the N-terminus, the EGF-like domain are linked via Ca2+ to the Gla-domain of the enzyme, which itself does not need Ca2+ for binding of C6PS Homo sapiens
Ca2+ required for binding of the EGF-like domains at the catalytic C-terminus and the N-terminus, the EGF-like domain are linked via Ca2+ to the Gla-domain of the enzyme, which itself does not need Ca2+ for binding of C6PS Bos taurus

Organism

Organism UniProt Comment Textmining
Bos taurus
-
-
-
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
native and recombinant wild-type, and mutant constructs expressed in 293 ells Homo sapiens
native and recombinant wild-type, and mutant constructs expressed in 293 ells Bos taurus

Reaction

Reaction Comment Organism Reaction ID
selective cleavage of Arg-/-Thr and then Arg-/-Ile bonds in prothrombin to form thrombin scutelarin has similar specificity Homo sapiens
a
selective cleavage of Arg-/-Thr and then Arg-/-Ile bonds in prothrombin to form thrombin scutelarin has similar specificity Bos taurus
a

Source Tissue

Source Tissue Comment Organism Textmining
plasma
-
 Homo sapiens
-
plasma
-
 Bos taurus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
N-alpha-benzyloxycarbonyl-D-arginyl-glycyl-L-arginine-4-nitroanilide + H2O i.e. S2765 Homo sapiens N-alpha-benzyloxycarbonyl-D-arginyl-glycyl-L-arginine + 4-nitroaniline
-
 ?
N-alpha-benzyloxycarbonyl-D-arginyl-glycyl-L-arginine-4-nitroanilide + H2O i.e. S2765 Bos taurus N-alpha-benzyloxycarbonyl-D-arginyl-glycyl-L-arginine + 4-nitroaniline
-
 ?
prothrombin + H2O
-
 Homo sapiens thrombin + ?
-
 ?
prothrombin + H2O
-
 Bos taurus thrombin + ?
-
 ?

Subunits

Subunits Comment Organism
More domain structure of the enzyme Homo sapiens
More domain structure of the enzyme Bos taurus

Synonyms

Synonyms Comment Organism
factor Xa
-
 Homo sapiens
factor Xa
-
 Bos taurus
More the enzyme belongs to the peptidase family S1, i.e. trypsin family Homo sapiens
More the enzyme belongs to the peptidase family S1, i.e. trypsin family Bos taurus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Homo sapiens
37
-
 assay at Bos taurus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.6
-
 assay at Homo sapiens
7.6
-
 assay at Bos taurus