Literature summary for 3.4.21.59 extracted from

Butterfield, J.H.; Weiler, D.A.; Hunt, L.W.; Wynn, S.R.; Roche, P.C.
Purification of tryptase from a human mast cell line (1990), J. Leukocyte Biol., 47, 409-419.

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Inhibitors

Inhibitors	Comment	Organism	Structure
Aprotinin	not	Homo sapiens
benzamidine	not	Homo sapiens
Dansyl-L-glutamyl-glycyl-L-arginine chloromethyl ketone	-	Homo sapiens
HCl	-	Homo sapiens
HgCl2	-	Homo sapiens
leupeptin	-	Homo sapiens
additional information	lima bean trypsin inhibitor; ovomucoid inhibitor; plasma (human)	Homo sapiens
N-tosyl-L-Lys chloromethyl ketone	-	Homo sapiens
PMSF	-	Homo sapiens
Soybean trypsin inhibitor	not	Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
32000	-	x * 32000 + x * 35000, human cell line HMC-1, SDS-PAGE	Homo sapiens
35000	-	x * 32000 + x * 35000, human cell line HMC-1, SDS-PAGE	Homo sapiens
150000	-	human mast cell line HMC-1, molecular sieve HPLC	Homo sapiens

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
-	Homo sapiens

Source Tissue

Source Tissue	Comment	Organism	Textmining
mast cell	cell line HMC-1 obtained from a patient with mast cell leukemia	Homo sapiens	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
Tosyl-Arg methyl ester + H2O	-	Homo sapiens	?	-	?

Subunits

Subunits	Comment	Organism
?	x * 32000 + x * 35000, human cell line HMC-1, SDS-PAGE	Homo sapiens

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
8.5	-	-	Homo sapiens

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Release 2023.1 (January 2023)