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Literature summary for 3.4.21.59 extracted from
- Tryptase from rat skin: purification and properties (1991), Biochemistry, 30, 4997-5007.
General Stability
| General Stability | Organism |
|---|---|
| stable to repeated freeze-thaw cycles | Rattus norvegicus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 4-Methylumbelliferyl 4-guanidinobenzoate | - |
Rattus norvegicus |  |
| 4-nitrophenyl 4-guanidinobenzoate | - |
Rattus norvegicus | |
| alpha1-antitrypsin | not | Rattus norvegicus | |
| antipain | - |
Rattus norvegicus | |
| Aprotinin | - |
Rattus norvegicus | |
| diisopropyl fluorophosphate | - |
Rattus norvegicus | |
| HgCl2 | - |
Rattus norvegicus | |
| leupeptin | - |
Rattus norvegicus | |
| additional information | lima bean trypsin inhibitor; ovomucoid | Rattus norvegicus | |
| N-tosyl-L-Lys chloromethyl ketone | - |
Rattus norvegicus | |
| Soybean trypsin inhibitor | not | Rattus norvegicus |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 145000 | - |
rat, gel filtration | Rattus norvegicus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Rattus norvegicus | - |
- |
- |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| glycoprotein | treatment of the enzyme with N-glycosidase decreases the subunit MW by 4000 | Rattus norvegicus |
| glycoprotein | putative glycosylation site at residue 21 | Rattus norvegicus |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| multiple isoelectric forms | Rattus norvegicus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| skin | - |
Rattus norvegicus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Rattus norvegicus |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 25°C, stable for at least 8 days | Rattus norvegicus |
| 4°C, stable for at least 6 months in buffer containing NaCl, rapid loss of activity without addition of NaCl | Rattus norvegicus |
| NaCl, 1 M, necessary for long term storage | Rattus norvegicus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | specificity overview: peptide 4-nitroanilides | Rattus norvegicus | ? | - |
? | |
| additional information | not: casein, hemoglobin, azocoll | Rattus norvegicus | ? | - |
? | |
| N-Benzoyl-Arg ethyl ester + H2O | - |
Rattus norvegicus | ? | - |
? | |
| neurotensin + H2O | - |
Rattus norvegicus | ? | - |
? | |
| Oxidized B-chain of insulin + H2O | cleavage at Arg8-Gly9-NH2, Arg8-Arg9- and Arg22-Gly23-bonds | Rattus norvegicus | ? | - |
? | |
| [Arg8]vasopressin + H2O | - |
Rattus norvegicus | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 30000-34000, rat | Rattus norvegicus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| additional information | - |
presence of 1 M NaCl improves thermal stability, heparin does not stabilize the enzyme against thermal denaturation | Rattus norvegicus |
| 25 | - |
pH 7.5, 30 min, stable up to | Rattus norvegicus |
| 30 | - |
pH 7.5, 30 min, 50% loss of activity | Rattus norvegicus |
| 35 | - |
pH 7.5, 30 min, complete loss of activity at 35°C or above | Rattus norvegicus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | 8 | - |
Rattus norvegicus |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 8.5 | no activity below pH 6 or above pH 8.5 | Rattus norvegicus |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 3.5 | 7.5 | 25°C, 2 h, relatively stable in this range, rapid loss of activity below and above | Rattus norvegicus |
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