Cloned (Comment) | Organism |
---|---|
gene lon, recombinant expression of C-terminally His6-tagged wild-type and mutant enzyme in Escherichia coli strain BL21 (DE3), expression of selenomethionine-labeled enzymes in Escherichia coli strain B834 (DE3) | Meiothermus taiwanensis |
Crystallization (Comment) | Organism |
---|---|
purified selenomethionine-labeled wild-type and mutant L91M/I359M enzymes alone and in complexes with inhibitors bortezomib, MG262, and clasto-lactacystin beta-lactone, sitting drop vapour diffusion technique, using a reservoir consisting of 10% 2-propanol, 100 mM dipotassium phosphate, 100 mM sodium citrate, pH 4.6, 22°C, X-ray diffraction structure determination and analysis at 1.9-2.1 A resolution | Meiothermus taiwanensis |
Protein Variants | Comment | Organism |
---|---|---|
I359M | site-directed mutagenesis | Meiothermus taiwanensis |
L91M | site-directed mutagenesis | Meiothermus taiwanensis |
L91M/I359M | site-directed mutagenesis, structure analysis with bound inhibitors | Meiothermus taiwanensis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
bortezomib | - |
Meiothermus taiwanensis | |
clasto-lactacystin beta-lactone | - |
Meiothermus taiwanensis | |
MG262 | - |
Meiothermus taiwanensis | |
additional information | binding structure of inhibitors, substrate-binding groove with covalently bound inhibitors, two peptidomimetics and one derived from a natural product, structural basis of inhibitor recognition, overview | Meiothermus taiwanensis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Meiothermus taiwanensis | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant C-terminally His6-tagged wild-type and mutant enzyme from Escherichia coli by nickel affinity chromatography and gel filtation | Meiothermus taiwanensis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
Abz-QLRSLNGEWRFAWFPAPEAV[Tyr(3-NO2)]A + H2O | i.e. F-beta20-Q peptide, the substrate is flanked by a fluorophore (Abz) and quencher (nitrotyrosine) pair | Meiothermus taiwanensis | ? | - |
? | |
additional information | the enzyme selectively degrades unfolded protein substrates in an ATP-independent manner, structural basis of substrate recognition, overview | Meiothermus taiwanensis | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
ATP-independent Lon-like protease | - |
Meiothermus taiwanensis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
additional information | an ATP-independent Lon-like protease | Meiothermus taiwanensis |