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Literature summary for 3.4.21.53 extracted from

  • Liao, J.H.; Ihara, K.; Kuo, C.I.; Huang, K.F.; Wakatsuki, S.; Wu, S.H.; Chang, C.I.
    Structures of an ATP-independent Lon-like protease and its complexes with covalent inhibitors (2013), Acta Crystallogr. Sect. D, 69, 1395-1402.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
gene lon, recombinant expression of C-terminally His6-tagged wild-type and mutant enzyme in Escherichia coli strain BL21 (DE3), expression of selenomethionine-labeled enzymes in Escherichia coli strain B834 (DE3) Meiothermus taiwanensis

Crystallization (Commentary)

Crystallization (Comment) Organism
purified selenomethionine-labeled wild-type and mutant L91M/I359M enzymes alone and in complexes with inhibitors bortezomib, MG262, and clasto-lactacystin beta-lactone, sitting drop vapour diffusion technique, using a reservoir consisting of 10% 2-propanol, 100 mM dipotassium phosphate, 100 mM sodium citrate, pH 4.6, 22°C, X-ray diffraction structure determination and analysis at 1.9-2.1 A resolution Meiothermus taiwanensis

Protein Variants

Protein Variants Comment Organism
I359M site-directed mutagenesis Meiothermus taiwanensis
L91M site-directed mutagenesis Meiothermus taiwanensis
L91M/I359M site-directed mutagenesis, structure analysis with bound inhibitors Meiothermus taiwanensis

Inhibitors

Inhibitors Comment Organism Structure
bortezomib
-
Meiothermus taiwanensis
clasto-lactacystin beta-lactone
-
Meiothermus taiwanensis
MG262
-
Meiothermus taiwanensis
additional information binding structure of inhibitors, substrate-binding groove with covalently bound inhibitors, two peptidomimetics and one derived from a natural product, structural basis of inhibitor recognition, overview Meiothermus taiwanensis

Organism

Organism UniProt Comment Textmining
Meiothermus taiwanensis
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant C-terminally His6-tagged wild-type and mutant enzyme from Escherichia coli by nickel affinity chromatography and gel filtation Meiothermus taiwanensis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Abz-QLRSLNGEWRFAWFPAPEAV[Tyr(3-NO2)]A + H2O i.e. F-beta20-Q peptide, the substrate is flanked by a fluorophore (Abz) and quencher (nitrotyrosine) pair Meiothermus taiwanensis ?
-
?
additional information the enzyme selectively degrades unfolded protein substrates in an ATP-independent manner, structural basis of substrate recognition, overview Meiothermus taiwanensis ?
-
?

Synonyms

Synonyms Comment Organism
ATP-independent Lon-like protease
-
Meiothermus taiwanensis

Cofactor

Cofactor Comment Organism Structure
additional information an ATP-independent Lon-like protease Meiothermus taiwanensis