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Literature summary for 3.4.21.53 extracted from
- The catalytic domain of E.coli Lon protease has a unique fold and a Ser-Lys dyad in the active site (2003), J. Biol. Chem., 279, 8140-8148.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| cDNAcloned and expressed in Escherichia coli Rosetta strain | Homo sapiens |
| cloning of the P-domain, expression as MBP fusion protein in Escherichia coli BL21 (DE3) | Escherichia coli |
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
| inactive Lon-S679A P-domain successfully crystallized by the hanging drop vapor diffusion method | Escherichia coli |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| S855A | site-directed mutagenesis | Homo sapiens |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| mitochondrion | - |
Homo sapiens | 5739 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 100000 | - |
6-7 * 100000, homo-oligomeric complex, SDS-PAGE | Homo sapiens |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | Escherichia coli | rapid proteolysis plays a major role in post-translational cellular control by the targeted degradation of short-lived regulatory proteins | ? | - |
? |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | - |
- |
- |
| Homo sapiens | - |
human | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| proteolytically inactive mutant Lon-S679A | Escherichia coli |
| recombinant enzyme | Homo sapiens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + H2O | - |
Homo sapiens | phosphate + ADP | - |
? | |
| casein + H2O | ATP dependent degradation> | Homo sapiens | ? | - |
? | |
| lambda phage DNA + H2O | - |
Homo sapiens | ? | - |
? | |
| additional information | participates directly in the metabolism of mitochodrial DNA | Homo sapiens | ? | - |
? | |
| additional information | rapid proteolysis plays a major role in post-translational cellular control by the targeted degradation of short-lived regulatory proteins | Escherichia coli | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| oligomer | - |
Escherichia coli |
| oligomer | 6-7 * 100000, homo-oligomeric complex, SDS-PAGE | Homo sapiens |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| lon protease | - |
Escherichia coli |
| lon protease | - |
Homo sapiens |
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Release 2023.1 (January 2023)
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