Cloned (Comment) | Organism |
---|---|
LonTK cloned and expressed in Escherichia coli | Thermococcus kodakarensis |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | membrane-bound | Thermococcus kodakarensis | 16020 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | - |
Thermococcus kodakarensis | |
Co2+ | supports the hydrolysis | Thermococcus kodakarensis | |
Mg2+ | - |
Thermococcus kodakarensis | |
Mn2+ | supports the hydrolysis | Thermococcus kodakarensis | |
Ni2+ | serves as a cofactor better than Mg2+ and Ca2+ | Thermococcus kodakarensis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
70000 | - |
SDS-PAGE | Thermococcus kodakarensis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermococcus kodakarensis | Q8NKS6 | KOD1, previously called Pyrococcus kodakaraensis | - |
Purification (Comment) | Organism |
---|---|
recombinant enzyme | Thermococcus kodakarensis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + H2O | - |
Thermococcus kodakarensis | phosphate + ADP | - |
? | |
glutaryl-Ala-Ala-Phe-4-methoxy-beta-naphthylamide + H2O | - |
Thermococcus kodakarensis | ? | - |
? | |
hemoglobin A + H2O | can degrade unfolded human hemoglobin A at 70°C either in presence or absence of ATP, at 37°C only in presence of ATP | Thermococcus kodakarensis | ? | - |
? | |
additional information | belonging to AAA+ superfamily | Thermococcus kodakarensis | ? | - |
? | |
ribulose-1,5-bisphosphate carboxylase/oxygenase + H2O | RubiscoTK | Thermococcus kodakarensis | ? | - |
? | |
succinyl-Phe-Leu-Phe-4-methoxy-beta-naphthylamide + H2O | - |
Thermococcus kodakarensis | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
archaeal Lon protease | - |
Thermococcus kodakarensis |
lon protease | - |
Thermococcus kodakarensis |
lonTK | - |
Thermococcus kodakarensis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | - |
peptide cleavage activity | Thermococcus kodakarensis |
95 | - |
ATPase activity | Thermococcus kodakarensis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9 | - |
- |
Thermococcus kodakarensis |