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Literature summary for 3.4.21.53 extracted from

  • Menon, A.S.; Goldberg, A.L.
    Protein substrates activate the ATP-dependent protease La by promoting nucleotide binding and release of bound ADP (1987), J. Biol. Chem., 262, 14929-14934.
    View publication on PubMed
Search for more literature for 3.4.21.53

Activating Compound

Activating Compound Comment Organism Structure
Adenyl-5'-yl imidodiphosphate i.e. AMP-PNP, activation Escherichia coli
Adenyl-5'-yl imidodiphosphate binding is stimulated by protein substrates Escherichia coli
Protein substrates protein substrates, e.g. denatured bovine serum albumin induce ADP-release and promote ATP-ADP-exchange Escherichia coli

Inhibitors

Inhibitors Comment Organism Structure
ADP ADP-release is rate-limiting step; binds 4 mol ADP per enzyme tetramer Escherichia coli

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ requirement Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
casein + H2O methylcasein Escherichia coli hydrolyzed casein
-
 ?
casein + H2O methylated alpha-casein Escherichia coli hydrolyzed casein
-
 ?
Denatured bovine serum albumin + H2O
-
 Escherichia coli ?
-
 ?
Globin + H2O
-
 Escherichia coli ?
-
 ?
additional information No substrates are native bovine serum albumin, hemoglobin Escherichia coli ?
-
 ?
additional information No substrates are native albumin Escherichia coli ?
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Escherichia coli

Cofactor

Cofactor Comment Organism Structure
ATP ATP-dependent protease Escherichia coli
ATP enzyme binds 4 mol ATP per tetramer Escherichia coli