Activating Compound | Comment | Organism | Structure |
---|---|---|---|
Adenyl-5'-yl imidodiphosphate | i.e. AMP-PNP, activation | Escherichia coli | |
Adenyl-5'-yl imidodiphosphate | competes for one of the ATP-high-affinity binding-sites | Escherichia coli | |
Adenyl-5'-yl imidodiphosphate | peptide hydrolysis | Escherichia coli | |
adenyl-5'-yl methylene monophosphonate | i.e. AMP-CPP, activation, competes for the two ATP-high affinity sites | Escherichia coli |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
ADP | ADP-binding in the presence of EDTA; kinetics; product inhibition | Escherichia coli | |
vanadate | decavanadate (not orthovanadate) | Escherichia coli |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | requirement | Escherichia coli | |
Mg2+ | 10 mM | Escherichia coli | |
Mn2+ | requirement, as Mn2+-ATP | Escherichia coli | |
Mn2+ | can replace Mg2+-ATP | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
carrying lon-gene on the chromosome | - |
Escherichia coli | - |
carrying lon-gene on plasmid pJMC40 | - |
Storage Stability | Organism |
---|---|
-70°C, in 40% glycerol, stable | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
casein + H2O | - |
Escherichia coli | hydrolyzed casein | - |
? | |
Glutaryl-Ala-Ala-Phe-methoxynaphthylamide + H2O | - |
Escherichia coli | Glutaryl-Ala-Ala-Phe + methoxynaphthylamine | - |
? | |
Succinyl-Phe-Ala-Phe-methoxynaphthylamide + H2O | - |
Escherichia coli | Succinyl-Phe-Ala-Phe + methoxynaphthylamine | - |
? |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | requirement | Escherichia coli | |
ATP | only phosphate is released after ATP hydrolysis | Escherichia coli | |
ATP | ADP remains on the enzyme | Escherichia coli | |
ATP | ATP-binding is reversible | Escherichia coli | |
ATP | ATP- or ATP-analog-binding in the presence of EDTA | Escherichia coli | |
ATP | ATP-dependent protease | Escherichia coli | |
ATP | enzyme binds 4 mol ATP per tetramer at ATP concentrations above 0.01 mM | Escherichia coli | |
ATP | enzyme binds 2 mol ATP/tetramer in the absence of divalent cations or at 0.01 mM ATP in the presence of Mn2+ or Mg2+ | Escherichia coli | |
ATP | diphosphate, adenyl-5'-yl methylene diphosphate, GTP, UTP and CTP at 1 mM have no effect on the binding of ATP to the enzyme | Escherichia coli |