Cloned (Comment) | Organism |
---|---|
- |
Lysobacter sp. |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ba2+ | 1 mM, 24% inhibition of amidolytic activity | Lysobacter sp. | |
diisopropyl fluorophosphate | 10 mM, almost complete inhibition of amidolytic activity | Lysobacter sp. | |
Nalpha-p-tosyl-L-Lys-chloromethylketone | 0.1 mM, almost complete inhibition of amidolytic activity | Lysobacter sp. | |
phenylmethanesulfonyl fluoride | 2 mM, almost complete inhibition of amidolytic activity | Lysobacter sp. | |
Zn2+ | 1 mM, 34% inhibition of amidolytic activity | Lysobacter sp. |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | - |
Lysobacter sp. | - |
- |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
27000 | - |
gel filtration | Lysobacter sp. |
27850 | - |
ion-spray mass spectrometry | Lysobacter sp. |
31000 | - |
1 * 31000, SDS-PAGE | Lysobacter sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Lysobacter sp. | Q9AQR5 | - |
- |
Lysobacter sp. IB-9374 | Q9AQR5 | - |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
proteolytic modification | the total precursor protein is 671 amino acids of which only 268 amino acids are in the finally processed exoenzyme | Lysobacter sp. |
Purification (Comment) | Organism |
---|---|
- |
Lysobacter sp. |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
culture medium | - |
Lysobacter sp. | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
5.6 | - |
- |
Lysobacter sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetyl-Lys-p-nitroaniline + H2O | - |
Lysobacter sp. | acetyl-Lys + p-nitroaniline | - |
? | |
amyloid beta-protein + H2O | only the Lys-X bond is hydrolyzed | Lysobacter sp. | ? | - |
? | |
amyloid beta-protein + H2O | only the Lys-X bond is hydrolyzed | Lysobacter sp. IB-9374 | ? | - |
? | |
benzoyl-Lys methyl ester + H2O | - |
Lysobacter sp. | benzoyl-Lys + methanol | - |
? | |
benzoyl-Lys methyl ester + H2O | - |
Lysobacter sp. IB-9374 | benzoyl-Lys + methanol | - |
? | |
benzoyl-Lys-p-nitroanilide + H2O | - |
Lysobacter sp. | benzoyl-Lys + p-nitroaniline | - |
? | |
benzoyl-Lys-p-nitroaniline + H2O | - |
Lysobacter sp. | benzoyl-Lys + p-nitroaniline | - |
? | |
benzoyl-Orn methyl ester + H2O | - |
Lysobacter sp. | benzoyl-Orn + methanol | - |
? | |
Glucagon + H2O | only the Lys-X bond is hydrolyzed | Lysobacter sp. | ? | - |
? | |
Glucagon + H2O | only the Lys-X bond is hydrolyzed | Lysobacter sp. IB-9374 | ? | - |
? | |
additional information | no activity with benzoyl-Arg-p-nitroanilide, Arg-p-nitroanilide, succinyl-Ala-p-nitroanilide, tert-butyloxycarbonyl-His-p-nitroanilide, acetyl-Phe-p-nitroanilide, benzoyl-Tyr-p-nitroanilide and acetyl-Leu-p-nitroanilide | Lysobacter sp. | ? | - |
? | |
additional information | no activity with benzoyl-Arg-p-nitroanilide, Arg-p-nitroanilide, succinyl-Ala-p-nitroanilide, tert-butyloxycarbonyl-His-p-nitroanilide, acetyl-Phe-p-nitroanilide, benzoyl-Tyr-p-nitroanilide and acetyl-Leu-p-nitroanilide | Lysobacter sp. IB-9374 | ? | - |
? | |
oxidized insulin B chain + H2O | only the Lys-X bond is hydrolyzed | Lysobacter sp. | ? | - |
? | |
oxidized insulin B chain + H2O | only the Lys-X bond is hydrolyzed | Lysobacter sp. IB-9374 | ? | - |
? | |
tosyl-Lys methyl ester + H2O | - |
Lysobacter sp. | tosyl-Lys + methanol | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 31000, SDS-PAGE | Lysobacter sp. |
Synonyms | Comment | Organism |
---|---|---|
LEP | - |
Lysobacter sp. |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
hydrolysis of benzoyl-Lys-p-nitroanilide | Lysobacter sp. |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | - |
pH 8.0, 30°C, stable | Lysobacter sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | 8.5 | esterolytic activity | Lysobacter sp. |
8.5 | 10.5 | caseinolytic activity | Lysobacter sp. |
9 | 9.6 | amidolytic activity | Lysobacter sp. |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Lysobacter sp. | isoelectric focusing | - |
6.8 |