Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ba2+ | competitive | Achromobacter lyticus | |
Cs+ | competitive | Achromobacter lyticus | |
diisopropylfluorophosphate | - |
Achromobacter lyticus | |
K+ | competitive | Achromobacter lyticus | |
Li+ | competitive | Achromobacter lyticus | |
Na+ | competitive | Achromobacter lyticus | |
Nalpha-p-tosyl-L-Lys-chloromethylketone | - |
Achromobacter lyticus | |
NH4+ | competitive | Achromobacter lyticus | |
phenylmethanesulfonyl fluoride | - |
Achromobacter lyticus | |
Rb+ | competitive | Achromobacter lyticus | |
Zn2+ | - |
Achromobacter lyticus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | - |
Achromobacter lyticus | - |
- |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
30000 | - |
1 * 30000, SDS-PAGE | Achromobacter lyticus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Achromobacter lyticus | - |
M497-1 | - |
Achromobacter lyticus M497-1 | - |
M497-1 | - |
Purification (Comment) | Organism |
---|---|
- |
Achromobacter lyticus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
culture filtrate | - |
Achromobacter lyticus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
casein + H2O | - |
Achromobacter lyticus | ? | - |
? | |
casein + H2O | - |
Achromobacter lyticus M497-1 | ? | - |
? | |
additional information | the enzyme cleaves only the Lys-X bond | Achromobacter lyticus | ? | - |
? | |
additional information | the enzyme cleaves only the Lys-X bond | Achromobacter lyticus M497-1 | ? | - |
? | |
N-benzoyl-L-Lys-p-nitroanilide + H2O | - |
Achromobacter lyticus | N-benzoyl-L-Lys + p-nitroaniline | - |
? | |
N-benzoyl-L-Lys-p-nitroanilide + H2O | - |
Achromobacter lyticus M497-1 | N-benzoyl-L-Lys + p-nitroaniline | - |
? | |
N-tosyl-L-Lys methyl ester + H2O | - |
Achromobacter lyticus | N-tosyl-L-Lys + methanol | - |
? | |
N-tosyl-L-Lys methyl ester + H2O | - |
Achromobacter lyticus M497-1 | N-tosyl-L-Lys + methanol | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 30000, SDS-PAGE | Achromobacter lyticus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
amidolytic activity, esterolytic activity and caseinolytic activity | Achromobacter lyticus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | - |
pH 9.0, 30 min, stable up to | Achromobacter lyticus |
60 | - |
30 min, complete loss of activity | Achromobacter lyticus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | 8.2 | esterolytic activity | Achromobacter lyticus |
7.8 | 8.2 | esterolytic activity | Achromobacter lyticus |
9 | 9.5 | amidolytic activity | Achromobacter lyticus |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
4 | 11 | 4°C, 24 h, stable | Achromobacter lyticus |