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Literature summary for 3.4.21.50 extracted from

  • Masaki, T.; Suzuki, H.; Soejima, M.
    Purification and some properties of Achromobacter protease Ia from Achromobacter lyticus M497-1 (1986), Agric. Biol. Chem., 50, 3087-3091.
No PubMed abstract available
Search for more literature for 3.4.21.50

General Stability

General Stability Organism
7 M urea, pH 8.0, 30°C, 20 min, 30% loss of activity Achromobacter lyticus

Inhibitors

Inhibitors Comment Organism Structure
Zn2+
-
 Achromobacter lyticus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.07
-
 N-benzoyl-L-Lys p-nitroanilide protease I Achromobacter lyticus
0.08
-
 Lys-p-nitroanilide protease I Achromobacter lyticus
0.083
-
 N-tosyl-L-Lys methyl ester protease Ia Achromobacter lyticus
0.09
-
 N-benzoyl-L-Lys p-nitroanilide protease Ia Achromobacter lyticus
0.1
-
 N-tosyl-L-Lys methyl ester protease I Achromobacter lyticus
0.11
-
 Lys-p-nitroanilide protease Ia Achromobacter lyticus
0.63
-
 N-benzoyl-L-Orn methyl ester protease Ia Achromobacter lyticus
1
-
 N-benzoyl-L-Orn methyl ester protease I Achromobacter lyticus
2.5
-
 N-tosyl-L-Arg methyl ester protease Ia Achromobacter lyticus
35.7
-
 N-tosyl-L-Arg methyl ester protease I Achromobacter lyticus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
27000
-
 gel filtration Achromobacter lyticus
30000
-
 gel filtration Achromobacter lyticus

Organism

Organism UniProt Comment Textmining
Achromobacter lyticus
-
 M497-1
-
Achromobacter lyticus
-
 protease I and protease Ia
-
Achromobacter lyticus M497-1
-
 M497-1
-

Purification (Commentary)

Purification (Comment) Organism
protease Ia Achromobacter lyticus

Source Tissue

Source Tissue Comment Organism Textmining
culture filtrate
-
 Achromobacter lyticus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Achromobacter lyticus

Storage Stability

Storage Stability Organism
4°C, pH 5.0-11.0, 24 h, stable Achromobacter lyticus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
B-chain of insulin + H2O protease I1 cleaves Lys29-Ala30 bond Achromobacter lyticus ?
-
 ?
B-chain of insulin + H2O protease I1 cleaves Lys29-Ala30 bond Achromobacter lyticus M497-1 ?
-
 ?
Lys-p-nitroanilide + H2O
-
 Achromobacter lyticus Lys + p-nitroaniline
-
 ?
Lys-p-nitroanilide + H2O
-
 Achromobacter lyticus M497-1 Lys + p-nitroaniline
-
 ?
additional information no hydrolysis of: N-benzoyl-L-Arg p-nitroanilide and Arg-p-nitroanilide Achromobacter lyticus ?
-
 ?
additional information no hydrolysis of: N-benzoyl-L-Arg p-nitroanilide and Arg-p-nitroanilide Achromobacter lyticus M497-1 ?
-
 ?
N-benzoyl-L-Lys-p-nitroanilide + H2O
-
 Achromobacter lyticus N-benzoyl-L-Lys + p-nitroaniline
-
 ?
N-benzoyl-L-Orn methyl ester + H2O
-
 Achromobacter lyticus N-benzoyl-L-Orn + methanol
-
 ?
N-tosyl-L-Arg methyl ester + H2O
-
 Achromobacter lyticus N-tosyl-L-Arg + methanol
-
 ?
N-tosyl-L-Arg methyl ester + H2O
-
 Achromobacter lyticus M497-1 N-tosyl-L-Arg + methanol
-
 ?
N-tosyl-L-Lys methyl ester + H2O
-
 Achromobacter lyticus N-tosyl-L-Lys + methanol
-
 ?
N-tosyl-L-Lys methyl ester + H2O
-
 Achromobacter lyticus M497-1 N-tosyl-L-Lys + methanol
-
 ?

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
40
-
 20 min, stable up to Achromobacter lyticus
60
-
 20 min, complete loss of activity Achromobacter lyticus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.8 8.2 esterolytic activity Achromobacter lyticus
8.8
-
 amidolytic activity Achromobacter lyticus

pH Stability

pH Stability pH Stability Maximum Comment Organism
5 11 4°C, 24 h, stable Achromobacter lyticus