Protein Variants | Comment | Organism |
---|---|---|
E229K | the mutation shifts the substrate specificity of thrombin by 130fold to favor the activation of the anticoagulant substrate protein C over the procoagulant substrate fibrinogen. The mutant enzyme is also less effective in activating platelets, is resistant to inhibition by antithrombin III and displays a prolonged half-life in plasma | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
antithrombin III | mutant enzymes E229A and E229K are not inhibited | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.002 | - |
protein C | mutant enzyme E229A | Homo sapiens | |
0.00215 | - |
protein C | mutant enzyme E229K | Homo sapiens | |
0.00295 | - |
D-Phe-L-pipecolyl-L-Arg-4-nitroanilide | wild-type enzyme | Homo sapiens | |
0.00312 | - |
protein C | mutant enzyme E229W | Homo sapiens | |
0.00316 | - |
fibrinogen | wild-type enzyme | Homo sapiens | |
0.00319 | - |
protein C | wild-type enzyme | Homo sapiens | |
0.0236 | - |
fibrinogen | mutant enzyme E229A | Homo sapiens | |
0.038 | - |
fibrinogen | mutant enzyme E229K | Homo sapiens | |
0.0502 | - |
D-Phe-L-pipecolyl-L-Arg-4-nitroanilide | mutant enzyme E229A | Homo sapiens | |
0.0528 | - |
fibrinogen | mutant enzyme E229W | Homo sapiens | |
0.0632 | - |
D-Phe-L-pipecolyl-L-Arg-4-nitroanilide | mutant enzyme E229K | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-Phe-L-pipecolyl-L-Arg-4-nitroanilide + H2O | - |
Homo sapiens | D-Phe-L-pipecolyl-L-Arg + 4-nitroaniline | - |
? | |
Fibrinogen + H2O | - |
Homo sapiens | ? | - |
? | |
protein C + H2O | the mutation E229K shifts the substrate specificity of thrombin by 130fold to favor the activation of the anticoagulant substrate protein C over the procoagulant substrate fibrinogen | Homo sapiens | ? | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.065 | - |
protein C | wild-type enzyme | Homo sapiens | |
0.148 | - |
protein C | mutant enzyme E229W | Homo sapiens | |
0.19 | - |
protein C | mutant enzyme E229A | Homo sapiens | |
0.21 | - |
protein C | mutant enzyme E229K | Homo sapiens | |
2.53 | - |
fibrinogen | mutant enzyme E229K | Homo sapiens | |
3.12 | - |
fibrinogen | mutant enzyme E229W | Homo sapiens | |
5.9 | - |
fibrinogen | wild-type enzyme | Homo sapiens | |
7.88 | - |
fibrinogen | mutant enzyme E229A | Homo sapiens |