Application | Comment | Organism |
---|---|---|
medicine | the enzyme can be engineered to function in vivo as a potent anticoagulant that may possess a superior therapeutic profile with reduced potential for bleeding complications | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
E229A | mutation substantially shifts thrombin's specificity in favour of the anticoagulant substrate, protein C | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.002 | - |
protein C | mutant enzyme E229A | Homo sapiens | |
0.00295 | - |
D-Phe-L-pipecolyl-L-Arg-4-nitroanilide | wild-type enzyme | Homo sapiens | |
0.0032 | - |
protein C | wild-type enzyme | Homo sapiens | |
0.00355 | - |
fibrinogen Aalpha chain | wild-type enzyme | Homo sapiens | |
0.0113 | - |
fibrinogen Aalpha chain | mutant enzyme E229A | Homo sapiens | |
0.0355 | - |
platelet thrombin receptor peptide | wild-type enzyme | Homo sapiens | |
0.0502 | - |
D-Phe-L-pipecolyl-L-Arg-4-nitroanilide | mutant enzyme E229A | Homo sapiens | |
0.181 | - |
platelet thrombin receptor peptide | mutant enzyme E229A | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
Fibrinogen + H2O | Homo sapiens | key regulator of blood coagulation | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
proteolytic modification | zymogen: prothrombin | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-Phe-L-pipecolyl-L-Arg-4-nitroanilide + H2O | - |
Homo sapiens | D-Phe-L-pipecolyl-L-Arg + 4-nitroaniline | - |
? | |
Fibrinogen + H2O | Aalpha chain | Homo sapiens | ? | - |
? | |
Fibrinogen + H2O | key regulator of blood coagulation | Homo sapiens | ? | - |
? | |
fibrinogen Aalpha chain + H2O | - |
Homo sapiens | ? | - |
? | |
platelet thrombin receptor peptide + H2O | - |
Homo sapiens | ? | - |
? | |
protein C + H2O | mutation E229A substantially shifts thrombin's specificity in favour of the anticoagulant substrate, protein C | Homo sapiens | ? | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.19 | - |
protein C | mutant enzyme E229A | Homo sapiens | |
0.65 | - |
protein C | wild-type enzyme | Homo sapiens | |
4.53 | - |
fibrinogen Aalpha chain | mutant enzyme E229A | Homo sapiens | |
25 | - |
platelet thrombin receptor peptide | mutant enzyme E229A | Homo sapiens | |
34 | - |
platelet thrombin receptor peptide | wild-type enzyme | Homo sapiens | |
38 | - |
D-Phe-L-pipecolyl-L-Arg-4-nitroanilide | mutant enzyme E229A | Homo sapiens | |
51 | - |
D-Phe-L-pipecolyl-L-Arg-4-nitroanilide | wild-type enzyme | Homo sapiens | |
57 | - |
fibrinogen Aalpha chain | wild-type enzyme | Homo sapiens |