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Literature summary for 3.4.21.5 extracted from
- Cloning, expression, purification and bioactivity evaluation of a thrombin-like enzyme from Deinagkistrodon acutus venom gland library (2018), Biotechnol. Lett., 40, 93-102 .
Application
| Application | Comment | Organism |
|---|---|---|
| medicine | fibrinogenolytic properties make the enzyme applicable for biochemical research and drug development on thrombolytic therapy | Deinagkistrodon acutus |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| a recombinant enzyme expression vector is constructed and the fused protein is expressed in Escherichia coli | Deinagkistrodon acutus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| additional information | no inhibition by EDTA | Deinagkistrodon acutus | |
| PMSF | 2 mM, complete inhibition | Deinagkistrodon acutus |  |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 49400 | - |
SDS-PAGE, recombinant enzyme containing the 20400 Da Trx tag | Deinagkistrodon acutus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Deinagkistrodon acutus | Q5I2C5 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Deinagkistrodon acutus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| venom | - |
Deinagkistrodon acutus | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| Fibrinogen Aalpha-chain + H2O | - |
Deinagkistrodon acutus | ? | - |
? | |
| additional information | the enzyme shows no factor XIII activation activity | Deinagkistrodon acutus | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 49400, SDS-PAGE, recombinant enzyme containing the 20400 Da Trx tag | Deinagkistrodon acutus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| TLE2 | - |
Deinagkistrodon acutus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 35 | 40 | - |
Deinagkistrodon acutus |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 15 | 50 | 15°C: about 45% of maximal activity, 50°C: about 50% of maximal activity | Deinagkistrodon acutus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | 8 | - |
Deinagkistrodon acutus |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | 9 | pH 5.5: about 60% of maximal activity, pH 9.0: about 60% of maximal activity | Deinagkistrodon acutus |
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