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Literature summary for 3.4.21.5 extracted from

  • Niu, W.; Chen, Z.; Bush-Pelc, L.A.; Bah, A.; Gandhi, P.S.; Di Cera, E.
    Mutant N143P reveals how Na+ activates thrombin (2009), J. Biol. Chem., 284, 36175-36185.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.4.21.5

Crystallization (Commentary)

Crystallization (Comment) Organism
mutant enzyme N143P is crystallized by the hanging drop vapor diffusion method, using 0.2 M NaNO3 and 20% (w/v) PEG3350 Homo sapiens

Protein Variants

Protein Variants Comment Organism
N143P t the mutant features no Na+-dependent enhancement of kcat yet binds Na+ with an affinity comparable to that of wild type Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00033
-
 D-Phe-Pro-Arg-4-nitroanilide wild type enzyme, in 5 mM Tris, 0.1% (w/v) PEG 8000, pH 8.0, 25°C, 200 mM NaCl Homo sapiens
0.00088
-
 D-Phe-Pro-Arg-4-nitroanilide wild type enzyme, in 5 mM Tris, 0.1% (w/v) PEG 8000, pH 8.0, 25°C, 200 mM choline chloride Homo sapiens
0.0029
-
 D-Phe-Pro-Arg-4-nitroanilide mutant enzyme N143P, in 5 mM Tris, 0.1% (w/v) PEG 8000, pH 8.0, 25°C, 200 mM NaCl Homo sapiens
0.004
-
 D-Phe-Pro-Lys-4-nitroanilide wild type enzyme, in 5 mM Tris, 0.1% (w/v) PEG 8000, pH 8.0, 25°C, 200 mM NaCl Homo sapiens
0.0064
-
 D-Phe-Pro-Lys-4-nitroanilide mutant enzyme N143P, in 5 mM Tris, 0.1% (w/v) PEG 8000, pH 8.0, 25°C, 200 mM NaCl Homo sapiens
0.013
-
 D-Phe-Pro-Arg-4-nitroanilide mutant enzyme N143P, in 5 mM Tris, 0.1% (w/v) PEG 8000, pH 8.0, 25°C, 200 mM choline chloride Homo sapiens
0.017
-
 D-Phe-Pro-Phe-4-nitroanilide wild type enzyme, in 5 mM Tris, 0.1% (w/v) PEG 8000, pH 8.0, 25°C, 200 mN NaCl Homo sapiens
0.033
-
 D-Phe-Pro-Phe-4-nitroanilide mutant enzyme N143P, in 5 mM Tris, 0.1% (w/v) PEG 8000, pH 8.0, 25°C, 200 mN NaCl Homo sapiens
0.04
-
 D-Phe-Pro-Phe-4-nitroanilide mutant enzyme N143P, in 5 mM Tris, 0.1% (w/v) PEG 8000, pH 8.0, 25°C, 200 mM choline chloride Homo sapiens
0.04
-
 D-Phe-Pro-Lys-4-nitroanilide wild type enzyme, in 5 mM Tris, 0.1% (w/v) PEG 8000, pH 8.0, 25°C, 200 mM choline chloride Homo sapiens
0.072
-
 D-Phe-Pro-Lys-4-nitroanilide mutant enzyme N143P, in 5 mM Tris, 0.1% (w/v) PEG 8000, pH 8.0, 25°C, 200 mM choline chloride Homo sapiens
0.11
-
 D-Phe-Pro-Phe-4-nitroanilide wild type enzyme, in 5 mM Tris, 0.1% (w/v) PEG 8000, pH 8.0, 25°C, 200 mM choline chloride Homo sapiens

Metals/Ions

Metals/Ions Comment Organism Structure
Na+ Na+ activates thrombin by securing the correct orientation of the Glu192-Gly193 peptide bond, which is likely flipped in the absence of cation Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
fibrinogen + H2O Homo sapiens
-
 fibrin + fibrinopeptide A + fibrinopeptide B
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-Phe-Pro-Arg-4-nitroanilide + H2O
-
 Homo sapiens D-Phe-Pro-Arg + 4-nitroaniline
-
 ?
D-Phe-Pro-Lys-4-nitroanilide + H2O
-
 Homo sapiens D-Phe-Pro-Lys + 4-nitroaniline
-
 ?
D-Phe-Pro-Phe-4-nitroanilide + H2O
-
 Homo sapiens D-Phe-Pro-Phe + 4-nitroaniline
-
 ?
fibrinogen + H2O
-
 Homo sapiens fibrin + fibrinopeptide A + fibrinopeptide B
-
 ?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.018
-
 D-Phe-Pro-Lys-4-nitroanilide mutant enzyme N143P, in 5 mM Tris, 0.1% (w/v) PEG 8000, pH 8.0, 25°C, 200 mM NaCl Homo sapiens
0.018
-
 D-Phe-Pro-Phe-4-nitroanilide mutant enzyme N143P, in 5 mM Tris, 0.1% (w/v) PEG 8000, pH 8.0, 25°C, 200 mN NaCl Homo sapiens
0.23
-
 D-Phe-Pro-Phe-4-nitroanilide mutant enzyme N143P, in 5 mM Tris, 0.1% (w/v) PEG 8000, pH 8.0, 25°C, 200 mM choline chloride Homo sapiens
0.26
-
 D-Phe-Pro-Lys-4-nitroanilide mutant enzyme N143P, in 5 mM Tris, 0.1% (w/v) PEG 8000, pH 8.0, 25°C, 200 mM choline chloride Homo sapiens
1.4
-
 D-Phe-Pro-Phe-4-nitroanilide wild type enzyme, in 5 mM Tris, 0.1% (w/v) PEG 8000, pH 8.0, 25°C, 200 mM choline chloride Homo sapiens
2 8 D-Phe-Pro-Lys-4-nitroanilide wild type enzyme, in 5 mM Tris, 0.1% (w/v) PEG 8000, pH 8.0, 25°C, 200 mM NaCl Homo sapiens
3.5
-
 D-Phe-Pro-Arg-4-nitroanilide wild type enzyme, in 5 mM Tris, 0.1% (w/v) PEG 8000, pH 8.0, 25°C, 200 mM choline chloride Homo sapiens
6
-
 D-Phe-Pro-Arg-4-nitroanilide mutant enzyme N143P, in 5 mM Tris, 0.1% (w/v) PEG 8000, pH 8.0, 25°C, 200 mM NaCl Homo sapiens
7.5
-
 D-Phe-Pro-Arg-4-nitroanilide mutant enzyme N143P, in 5 mM Tris, 0.1% (w/v) PEG 8000, pH 8.0, 25°C, 200 mM choline chloride Homo sapiens
8.3
-
 D-Phe-Pro-Phe-4-nitroanilide wild type enzyme, in 5 mM Tris, 0.1% (w/v) PEG 8000, pH 8.0, 25°C, 200 mN NaCl Homo sapiens
12
-
 D-Phe-Pro-Lys-4-nitroanilide wild type enzyme, in 5 mM Tris, 0.1% (w/v) PEG 8000, pH 8.0, 25°C, 200 mM choline chloride Homo sapiens
29
-
 D-Phe-Pro-Arg-4-nitroanilide wild type enzyme, in 5 mM Tris, 0.1% (w/v) PEG 8000, pH 8.0, 25°C, 200 mM NaCl Homo sapiens