Crystallization (Comment) | Organism |
---|---|
purified enzyme, hanging drop vapour diffusion metod, 20°C, 0.002 ml of protein in 10 mM Tris-HCl, pH 8.0, and 50 mM NaCl, is mixed with 0.002 ml of reservoir solution containing 0.1 M sodium citrate, pH 5.6, 14-16% w/v PEG 4000, and 20% w/v 2-propanol, and equilibration against 0.1 ml reservoir solution, X-ray diffraction structure determination and analysis at 2.8 A resolution, molecular replacement method | Bos taurus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
activated protein C | activated protein C has a regulatory function in inhibiting thrombin activation, overview | Bos taurus | |
Thrombomodulin | complex formation on endothelial cell surfaces blocks thrombin activity | Bos taurus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
factor V + H2O | Bos taurus | activation | activated factor V + ? | - |
? | |
factor VIII + H2O | Bos taurus | activation | activated factor VIII + ? | - |
? | |
factor XI + H2O | Bos taurus | activation | activated factor XI + ? | - |
? | |
factor XII + H2O | Bos taurus | activated factor XII cross-links fibrin molecules and stabilizes the fibrin clot | activated factor XII + ? | - |
? | |
fibrinogen + H2O | Bos taurus | the enzyme mediates the conversion of fibrinogen to fibrin | fibrin + ? | - |
? | |
additional information | Bos taurus | thrombin is a multifunctional trypsin-like protease that plays a role in the blood coagulation system, stimulates platelet aggregation, and promotes its own generation through the activation of factor XI and cofactors V and VII | ? | - |
? | |
protease-activated receptor + H2O | Bos taurus | activation | ? | - |
? | |
protein C zymogen + H2O | Bos taurus | activated protein C has a regulatory function in inhibiting thrombin activation, overview | activated protein C + propeptide | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bos taurus | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
commercial preparation | - |
Bos taurus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
factor V + H2O | activation | Bos taurus | activated factor V + ? | - |
? | |
factor VIII + H2O | activation | Bos taurus | activated factor VIII + ? | - |
? | |
factor XI + H2O | activation | Bos taurus | activated factor XI + ? | - |
? | |
factor XII + H2O | - |
Bos taurus | activated factor XII + ? | - |
? | |
factor XII + H2O | activated factor XII cross-links fibrin molecules and stabilizes the fibrin clot | Bos taurus | activated factor XII + ? | - |
? | |
fibrinogen + H2O | - |
Bos taurus | fibrin + ? | - |
? | |
fibrinogen + H2O | the enzyme mediates the conversion of fibrinogen to fibrin | Bos taurus | fibrin + ? | - |
? | |
additional information | thrombin is a multifunctional trypsin-like protease that plays a role in the blood coagulation system, stimulates platelet aggregation, and promotes its own generation through the activation of factor XI and cofactors V and VII | Bos taurus | ? | - |
? | |
protease-activated receptor + H2O | activation | Bos taurus | ? | - |
? | |
protease-activated receptor + H2O | activation | Bos taurus | activated protease-activated receptor + ? | - |
? | |
protein C zymogen + H2O | activation | Bos taurus | activated protein C + propeptide | - |
? | |
protein C zymogen + H2O | activated protein C has a regulatory function in inhibiting thrombin activation, overview | Bos taurus | activated protein C + propeptide | - |
? |