Literature summary for 3.4.21.5 extracted from

Dullweber, F.; Stubbs, M.T.; Musil, D.; Sturzebecher, J.; Klebe, G.
Factorising ligand affinity: a combined thermodynamic and crystallographic study of trypsin and thrombin inhibition (2001), J. Mol. Biol., 313, 593-614.

Search for more literature for 3.4.21.5

Crystallization (Commentary)

Crystallization (Comment)	Organism
enzyme complexed with hirugen, 5.5 mg/ml in 24-30% PEG 4000, 0.1 M sodium phosphate, pH 7.3, 20°C, vapour diffusion method, single crystals are soaked overnight in 30% PEG 4000, 0.1 M sodium phosphate, pH 7.3, and 0.5 mg/ml inhibitor, X-ray diffraction structure determination and analysis	Homo sapiens

Inhibitors

Inhibitors	Comment	Organism	Structure
CRC 220	binding mode to the enzyme, crystal structure	Homo sapiens
inogatran	binding mode to the enzyme, crystal structure	Homo sapiens
melagatran	binding mode to the enzyme, crystal structure	Homo sapiens
additional information	inhibition mechanism	Homo sapiens
napsagatran	binding mode to the enzyme, crystal structure	Bos taurus
napsagatran	binding mode to the enzyme, crystal structure	Homo sapiens
napsagatran ethyl ester	binding mode to the enzyme, crystal structure	Bos taurus
napsagatran ethyl ester	binding mode to the enzyme, crystal structure	Homo sapiens

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
additional information	-	additional information	thermodynamic analysis of enzyme structure	Homo sapiens
additional information	-	additional information	thermodynamic analysis of enzyme structure	Bos taurus

Organism

Organism	UniProt	Comment	Textmining
Bos taurus	P00735	-	-
Homo sapiens	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
-	Bos taurus

Source Tissue

Source Tissue	Comment	Organism	Textmining
commercial preparation	-	Homo sapiens	-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
0.019	-	purified enzyme	Bos taurus

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
additional information	determination of enzyme activity by active site titration with 4-nitrophenyl guanidino-benzoate	Bos taurus	?	-	?

Synonyms

Synonyms	Comment	Organism
alpha-thrombin	-	Homo sapiens
alpha-thrombin	-	Bos taurus

Ki Value [mM]

Ki Value [mM]	Ki Value maximum [mM]	Inhibitor	Comment	Organism	Structure
additional information	-	additional information	inhibition kinetics for napsagatran and napsagatran ethyl ester in presence of NaCl or KCl and at 4 different temperatures	Bos taurus

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Release 2023.1 (January 2023)