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Literature summary for 3.4.21.5 extracted from

  • Lopez, M.; Mende, K.; Steinmetzer, T.; Nowak, G.
    Cloning, purification and biochemical characterization of dipetarudin, a new chimeric thrombin inhibitor (2003), J. Chromatogr. B, 786, 73-80.
    View publication on PubMed

Application

Application Comment Organism
medicine enzyme is a target for development of inhibitors in antithrombotic therapy Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
dipetalogastin II strong inhibitor, fron the assassin bug Dipetalogaster maximus Homo sapiens
dipetarudin cloning and purification of the chimeric inhibitor composed of the N-terminal head structure of dipetalogastin II and the exosite 1 blcking segment of hirudin, connected through a five glycine linker, MW 7560 Homo sapiens
Hirudin blocks exosite 1 Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Reaction

Reaction Comment Organism Reaction ID
selective cleavage of Arg-/-Gly bonds in fibrinogen to form fibrin and release fibrinopeptides A and B serine protease Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
commercial preparation
-
Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-phenylalanyl-pipecolyl-L-arginine-4-nitroanilide + H2O i.e. S2238, synthetic chromogenic substrate Homo sapiens D-phenylalanyl-pipecolyl-L-arginine + 4-nitroaniline
-
?

Synonyms

Synonyms Comment Organism
alpha-thrombin
-
Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
assay at Homo sapiens

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.0000000000493
-
dipetalogastin II pH 8.0, 37°C Homo sapiens
0.000000000237
-
Hirudin pH 8.0, 37°C Homo sapiens
0.000000000446
-
dipetarudin pH 8.0, 37°C Homo sapiens