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Literature summary for 3.4.21.41 extracted from
- The human complement system serine proteases C1bar and C1sbar and their proenzymes (1981), Methods Enzymol., 80, 26-42.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 4-Nitrophenyl-4-guanidinobenzoate | - |
Homo sapiens |  |
| C1bar-inhibitor | irreversible | Homo sapiens | |
| diisopropyl fluorophosphate | - |
Homo sapiens | |
| leupeptin | competitive | Homo sapiens | |
| NaCl | above 200 mM | Homo sapiens | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 20 | - |
acetyl-Gly-Lys methyl ester | - |
Homo sapiens | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 85000 | - |
2 * 85000, the C1rbar subunits consist of one polypeptide chain of 56000 Da, A-chain, that is disulfide-linked to a 27000 Da B-chain, SDS-PAGE | Homo sapiens |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| complement component C1s | Homo sapiens | activates the proenzyme form of C1s by limited proteolysis | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
- |
- |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| proteolytic modification | the proenzyme is C1r | Homo sapiens |
| proteolytic modification | activation of the proenzyme C1r can be mimicked under certain conditions by digestion of C1r with trypsin or plasmin | Homo sapiens |
| side-chain modification | C1rbar contains about 40 carbohydrate residues per molecule | Homo sapiens |
| side-chain modification | the zymogen C1r is a glycoprotein | Homo sapiens |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Homo sapiens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| acetyl-Arg methyl ester + H2O | - |
Homo sapiens | acetyl-Arg + methanol | - |
? | |
| complement component C1s | activates the proenzyme form of C1s by limited proteolysis | Homo sapiens | ? | - |
? | |
| complement component C1s + H2O | cleavage of a single Arg-Ile or Lys-Ile bond in C1s | Homo sapiens | complement component C1sbar | - |
? | |
| N-acetyl-Gly-Lys methyl ester + H2O | - |
Homo sapiens | N-acetyl-Gly-Lys + methanol | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 85000, the C1rbar subunits consist of one polypeptide chain of 56000 Da, A-chain, that is disulfide-linked to a 27000 Da B-chain, SDS-PAGE | Homo sapiens |
| More | on activation the single polypeptide chain of C1r is cleaved probably at a single position, the C1rbar subunits consist of 1 polypeptide chain of 56000 Da, A-chain, that is disulfide-linked to a 27000 Da B-chain | Homo sapiens |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | 8.5 | - |
Homo sapiens |
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