Inhibitors | Comment | Organism | Structure |
---|---|---|---|
clispin | a Clitocybe nebularis trypsin inhibitor variant, recombinantly expressed in Escherichia coli, highly specific towards trypsin, fast-acting, and tight-binding. Molecular cloning of the gene and cDNA encoding cnispin, overview | Homo sapiens | |
Clitocybe nebularis trypsin inhibitor | CnSPI, several inhibitor variants, separation by trypsin affinity chromatography. Possible regulatory role for CnSPIs in the endogenous proteolytic system of Clitocybe nebularis. Isolation from frozen fruiting bodies or basidiocarps | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
lymphocyte | peripheral blood | Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
N-benzoyl-DL-arginine 4-nitroanilide + H2O | - |
Homo sapiens | N-benzoyl-DL-arginine + 4-nitroaniline | - |
? | |
N-benzyloxycarbonyl-Phe-Arg-7-amido-4-methylcoumarin + H2O | - |
Homo sapiens | N-benzyloxycarbonyl-Phe-Arg + 7-amino-4-methylcoumarin | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at | Homo sapiens |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | pseudo-first-order inhibition kinetics for trypsin, overview | Homo sapiens | |
0.0000031 | - |
clispin | pH 8.0, 37°C | Homo sapiens |