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Literature summary for 3.4.21.4 extracted from

  • Bougatef, A.; Balti, R.; Nasri, R.; Jellouli, K.; Souissi, N.; Nasri, M.
    Biochemical properties of anionic trypsin acting at high concentration of NaCl purified from the intestine of a carnivorous fish: smooth hound (Mustelus mustelus) (2010), J. Agric. Food Chem., 58, 5763-5769.
    View publication on PubMed
Search for more literature for 3.4.21.4

Inhibitors

Inhibitors Comment Organism Structure
benzamidine 54% inhibition at 5 mM Mustelus mustelus
additional information no or poor inhibition by pepstatin A, EDTA, 2-mercaptoethanol, and N-p-tosyl-L-phenylalanine chloromethyl ketone Mustelus mustelus
N-p-tosyl-L-lysine chloromethyl ketone 88% inhibition at 5 mM Mustelus mustelus
NaCl enzyme activity continuously decreases to 50% of maximal activity with increasing NaCl concentration of 0-30% Mustelus mustelus
PMSF 89% inhibition at 5 mM Mustelus mustelus
Soybean trypsin inhibitor 91.5% inhibition at 1 mg/ml Mustelus mustelus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.156
-
 Nalpha-4-tosyl-L-arginine methyl ester pH 8.5, 25°C Mustelus mustelus
0.387
-
 N-benzoyl-DL-arginine 4-nitroanilide pH 8.5, 25°C Mustelus mustelus

Organism

Organism UniProt Comment Textmining
Mustelus mustelus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
native enzyme 65fold from intestine by ammonium sulfate fractionation, gel filtration, and anion exchange chromatography Mustelus mustelus

Source Tissue

Source Tissue Comment Organism Textmining
intestine
-
 Mustelus mustelus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
21.65
-
 purified enzyme, pH 8.5, 25°C Mustelus mustelus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
N-benzoyl-DL-arginine 4-nitroanilide + H2O
-
 Mustelus mustelus N-benzoyl-DL-arginine + 4-nitroaniline
-
 ?
Nalpha-4-tosyl-L-arginine methyl ester + H2O
-
 Mustelus mustelus Nalpha-4-tosyl-L-arginine + methanol
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
-
 Mustelus mustelus

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
20 60 profile, overview, inactivation at 70°C Mustelus mustelus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
40
-
 purified enzyme, 1 h, highly stable up to, unstable above Mustelus mustelus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.62
-
 N-benzoyl-DL-arginine 4-nitroanilide pH 8.5, 25°C Mustelus mustelus
59.15
-
 Nalpha-4-tosyl-L-arginine methyl ester pH 8.5, 25°C Mustelus mustelus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.5
-
-
 Mustelus mustelus

pH Range

pH Minimum pH Maximum Comment Organism
4 12 profile, overview Mustelus mustelus

pH Stability

pH Stability pH Stability Maximum Comment Organism
5 12 purified enzyme, 1 h, extremely stable at pH 7.0-9.0, profile, overview Mustelus mustelus