General Stability | Organism |
---|---|
Ca2+ stabilizes the purified enzyme | Albatrossia pectoralis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
EDTA | 10% inhibition at 2 mM | Albatrossia pectoralis | |
additional information | no inhibition by E-64, N-ethylmaleimide, iodoacetic acid, pepstatin A, and Nalpha-4-tosyl-L-Phe-chloromethylketone | Albatrossia pectoralis | |
NaCl | enzyme activity continuously decreases with increasing NaCl concentration of 0-30% | Albatrossia pectoralis | |
Nalpha-tosyl-L-Lys-chloromethyl ketone | 91% inhibition at 1 mM | Albatrossia pectoralis | |
Soybean trypsin inhibitor | complete inhibition at 1 mg/ml | Albatrossia pectoralis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.15 | - |
Nalpha-4-tosyl-L-arginine methyl ester | pH 8.5, 45°C | Albatrossia pectoralis |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | stabilizes the purified enzyme | Albatrossia pectoralis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
24000 | - |
gel filtration | Albatrossia pectoralis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Albatrossia pectoralis | - |
- |
- |
Purification (Comment) | Organism |
---|---|
native enzyme 89fold from pyloric ceca by ammonium sulfate fractionation, gel filtration, anion exchange chromatography, and again gel filtration | Albatrossia pectoralis |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
cecum | - |
Albatrossia pectoralis | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
8.9 | - |
purified enzyme, pH 8.5, 45°C | Albatrossia pectoralis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
Nalpha-4-tosyl-L-arginine methyl ester + H2O | - |
Albatrossia pectoralis | Nalpha-4-tosyl-L-arginine + methanol | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 24000, SDS-PAGE | Albatrossia pectoralis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | - |
- |
Albatrossia pectoralis |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | 60 | activity range, 20-25% of maximal activity at 20°C and 60°C, profile, overview | Albatrossia pectoralis |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
purified enzyme, unstable above, 50% remaining activity at 60°C, inactivation at 80°C | Albatrossia pectoralis |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
210 | - |
Nalpha-4-tosyl-L-arginine methyl ester | pH 8.5, 45°C | Albatrossia pectoralis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.5 | - |
- |
Albatrossia pectoralis |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5 | 10 | activity range, 20% of maximal activity at pH 5.0 and pH 10.0, profile, overview | Albatrossia pectoralis |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
6 | 11 | purified enzyme, stable, unstable below | Albatrossia pectoralis |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1400 | - |
Nalpha-4-tosyl-L-arginine methyl ester | pH 8.5, 45°C | Albatrossia pectoralis |