Any feedback?
Literature summary for 3.4.21.4 extracted from
- Purification and characteristics of trypsins from cold-zone fish, Pacific cod (Gadus macrocephalus) and saffron cod (Eleginus gracilis) (2009), Food Chem., 116, 611-616.
General Stability
| General Stability | Organism |
|---|---|
| Ca2+ stabilizes | Gadus macrocephalus |
| Ca2+ stabilizes | Eleginus gracilis |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| additional information | no or poor inhibition by E-64, N-ethylmaleimide, iodoacetic acid, TPCK, pepstatin A, and EDTA | Eleginus gracilis | |
| additional information | no or poor inhibition by E-64, N-ethylmaleimide, iodoacetic acid, TPCK, pepstatin A, and EDTA | Gadus macrocephalus | |
| Nalpha-tosyl-L-Lys-chloromethylketone | 79% inhibition at 5 mM | Eleginus gracilis |  |
| Nalpha-tosyl-L-Lys-chloromethylketone | 89% inhibition at 5 mM | Gadus macrocephalus | |
| Soybean trypsin inhibitor | 84% inhibition at 1 mg/ml | Eleginus gracilis | |
| Soybean trypsin inhibitor | 80% inhibition at 1 mg/ml | Gadus macrocephalus |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | stabilizes | Gadus macrocephalus | |
| Ca2+ | stabilizes | Eleginus gracilis | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 24000 | - |
x * 24000, SDS-PAGE | Gadus macrocephalus |
| 24000 | - |
x * 24000, SDS-PAGE | Eleginus gracilis |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Eleginus gracilis | - |
- |
- |
| Gadus macrocephalus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| native enzyme 28fold from pyloric ceca to homogeneity by gel filtration | Eleginus gracilis |
| native enzyme 33fold from pyloric ceca to homogeneity by gel filtration | Gadus macrocephalus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| cecum | - |
Gadus macrocephalus | - |
| cecum | - |
Eleginus gracilis | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 18 | - |
purified enzyme, pH 8.0, 50°C | Eleginus gracilis |
| 24 | - |
purified enzyme, pH 8.0, 50°C | Gadus macrocephalus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | the enzyme from catayzes also the reverse reaction and synthesizes Nalpha-(tert-butoxycarbonyl)-L-alanyl-L-alanine-4-nitroanilide from Nalpha-(tert-butoxycarbonyl)-L-alanine-4-guanidinophenyl ester as acyl donor and L-alanine-p-nitroanilide (L-Ala-pNA) as acyl acceptor, overview | Gadus macrocephalus | ? | - |
? | |
| Nalpha-(tert-butoxycarbonyl)-L-alanyl-L-alanine-4-nitroanilide + H2O | - |
Gadus macrocephalus | Nalpha-(tert-butoxycarbonyl)-L-alanyl-L-alanine + 4-nitroaniline | - |
? | |
| Nalpha-p-tosyl-L-arginine methyl ester + H2O | - |
Gadus macrocephalus | ? | - |
? | |
| Nalpha-p-tosyl-L-arginine methyl ester + H2O | - |
Eleginus gracilis | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 24000, SDS-PAGE | Gadus macrocephalus |
| ? | x * 24000, SDS-PAGE | Eleginus gracilis |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| EG-T | - |
Eleginus gracilis |
| GM-T | - |
Gadus macrocephalus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
- |
Gadus macrocephalus |
| 50 | - |
- |
Eleginus gracilis |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 20 | 70 | activity range | Gadus macrocephalus |
| 20 | 70 | activity range | Eleginus gracilis |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
purified enzyme, unstable above | Gadus macrocephalus |
| 30 | - |
purified enzyme, unstable above | Eleginus gracilis |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
- |
Gadus macrocephalus |
| 8 | - |
- |
Eleginus gracilis |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 7 | 9 | high activity within this range | Gadus macrocephalus |
| 7 | 9 | high activity within this range | Eleginus gracilis |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 10 | purified enzyme, unstable below, completely stable at pH 6.0-10.0 for 30 min | Eleginus gracilis |
| 5 | 10 | purified enzyme, unstable below, completely stable at pH 7.0-10.0 for 30 min | Gadus macrocephalus |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
