Literature summary for 3.4.21.4 extracted from

Tubio, G.; Pico, G.A.; Nerli, B.B.
Extraction of trypsin from bovine pancreas by applying polyethyleneglycol/sodium citrate aqueous two-phase systems (2009), J. Chromatogr. B, 877, 115-120.

Organism

Organism	UniProt	Comment	Textmining
Bos taurus	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
liquid-liquid extraction with polyethyleneglycol/sodium citrate aqueous two-phase systems, aqueous two-phase systems formed by PEG3350 and NaCit pH 5.2 show the best separation capability, the addition of NaCl up to a final concentration of 7% (w/w) and the decrease of top/bottom volume ratio to 0.1 leads to the recovery of 60% of pancreatic trypsin in a concentrated form in the top phase with a 3fold purification	Bos taurus

Purification (Comment)

Organism

liquid-liquid extraction with polyethyleneglycol/sodium citrate aqueous two-phase systems, aqueous two-phase systems formed by PEG3350 and NaCit pH 5.2 show the best separation capability, the addition of NaCl up to a final concentration of 7% (w/w) and the decrease of top/bottom volume ratio to 0.1 leads to the recovery of 60% of pancreatic trypsin in a concentrated form in the top phase with a 3fold purification

Bos taurus

Source Tissue

Source Tissue	Comment	Organism	Textmining
pancreas	-	Bos taurus	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
N-alpha-benzoyl-L-Arg-4-nitroanilide + H2O	-	Bos taurus	N-alpha-benzoyl-L-Arg + 4-nitroaniline	-	?

Synonyms

Synonyms	Comment	Organism
TRP	-	Bos taurus

pH Stability

pH Stability	pH Stability Maximum	Comment	Organism
5.2	-	pH 5.2 prevents autocatalytic hydrolysis of trypsin	Bos taurus

Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.

Release 2023.1 (January 2023)