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Literature summary for 3.4.21.4 extracted from

  • Hehemann, J.H.; Redecke, L.; Murugaiyan, J.; von Bergen, M.; Betzel, C.; Saborowski, R.
    Autoproteolytic stability of a trypsin from the marine crab Cancer pagurus (2008), Biochem. Biophys. Res. Commun., 370, 566-571.
    View publication on PubMed
Search for more literature for 3.4.21.4

General Stability

General Stability Organism
isozyme TryIII displays high resistance against autoproteolysis with a low susceptibility of the N-terminal domain towards autolysis Cancer pagurus

Inhibitors

Inhibitors Comment Organism Structure
4-(2-aminoethyl)-benzensulfonylfluoride isozyme TryIII activity is completely (more than 95%) inhibited by 4-(2-aminoethyl)-benzensulfonylfluoride Cancer pagurus
additional information aspartate (PepA) and cysteine (E64) protease inhibitors as well as the chymotrypsin inhibitor tosyl-L-phenylalanine chloromethylketone have no effect on enzymatic activity of isozyme TryIII Cancer pagurus
N-alpha-tosyl-L-lysine chlormethylketone less than 50% inhibition of isozyme TryIII by the trypsin-specific inhibitor N-alpha-tosyl-L-lysine chlormethylketone Cancer pagurus
Soybean trypsin inhibitor isozyme TryIII activity is completely (more than 95%) inhibited by soybean trypsin inhibitor Cancer pagurus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
25700
-
 isozyme TryIII, calculated from amino acid sequence Cancer pagurus
26000
-
 isozyme TryIII, SDS-PAGE Cancer pagurus

Organism

Organism UniProt Comment Textmining
Cancer pagurus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
NAP-10 Sephadex G25 gel filtration and UNO Q6 column chromatography Cancer pagurus

Source Tissue

Source Tissue Comment Organism Textmining
gastric juice
-
 Cancer pagurus
-
hepatopancreas
-
 Cancer pagurus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
N-alpha-benzoyl-L-Arg-4-nitroanilide + H2O
-
 Cancer pagurus N-alpha-benzoyl-L-Arg + 4-nitroaniline
-
 ?

Synonyms

Synonyms Comment Organism
TryI isozyme Cancer pagurus
TryII isozyme Cancer pagurus
TryIII isozyme Cancer pagurus

pI Value

Organism Comment pI Value Maximum pI Value
Cancer pagurus isozyme TryIII, calculated from amino acid sequence
-
 4.4