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Literature summary for 3.4.21.4 extracted from
- Novel carbohydrate-binding activity of pancreatic trypsins to N-linked glycans of glycoproteins (2006), J. Biol. Chem., 281, 8528-8538.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| methyl-alpha-D-galactoside | 1.2fold enhancement of activity at 0.2 M | Sus scrofa |  |
| methyl-alpha-D-galactoside | 1.2fold enhancement of activity at 0.2 M | Bos taurus | |
| methyl-alpha-D-mannoside | 1.4fold enhancement of activity at 0.2 M | Sus scrofa | |
| methyl-alpha-D-mannoside | 1.4fold enhancement of activity at 0.2 M | Bos taurus | |
| additional information | activity is not enhanced in 0.2 M lactose | Sus scrofa | |
| additional information | activity is not enhanced in 0.2 M lactose | Bos taurus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| ovomucoid | - |
Bos taurus | |
| ovomucoid | - |
Sus scrofa |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1.26 | - |
N-alpha-benzoyl-L-arginine ethyl ester | in 20 mM TBS (pH 7.6) for 10 min at 25°C, in the absence of sugars or glycoproteins | Sus scrofa | |
| 2.81 | - |
N-alpha-benzoyl-L-arginine ethyl ester | in 20 mM TBS (pH 7.6) for 10 min at 25°C, in the presence of 0.2 M methyl-alpha-D-galactoside | Sus scrofa | |
| 3.2 | - |
N-alpha-benzoyl-L-arginine ethyl ester | in 20 mM TBS (pH 7.6) for 10 min at 25°C, in the presence of 0.2 M methyl-alpha-D-mannoside | Sus scrofa | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bos taurus | - |
- |
- |
| Sus scrofa | - |
- |
- |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| pancreas | - |
Sus scrofa | - |
| pancreas | - |
Bos taurus | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| N-alpha-benzoyl-DL-arginine-p-nitroanilide + H2O | - |
Sus scrofa | N-alpha-benzoyl-DL-arginine + p-nitroaniline | - |
? | |
| N-alpha-benzoyl-DL-arginine-p-nitroanilide + H2O | - |
Bos taurus | N-alpha-benzoyl-DL-arginine + p-nitroaniline | - |
? | |
| N-alpha-benzoyl-L-arginine ethyl ester + H2O | - |
Bos taurus | ? | - |
? | |
| N-alpha-benzoyl-L-arginine ethyl ester + H2O | - |
Sus scrofa | N-alpha-benzoyl-L-arginine + ethanol | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| BPT | - |
Bos taurus |
| PPT | - |
Sus scrofa |
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