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Literature summary for 3.4.21.4 extracted from
- Characteristics of trypsin from the pyloric ceca of walleye pollock (Theragra chalcogramma) (2007), Food Chem., 106, 194-199.
No PubMed abstract available
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| additional information | 1-(L-trans-epoxysuccinyl-leucylamino)-4-guanidinobutane, N-ethylmaleimide, iodoacetic acid, chymotrypsin, pepstatin A, and EDTA have no inhibitory effect | Gadus chalcogrammus | |
| N-tosyl-L-lysine-chloromethyl ketone | 82% inhibition at 5 mM | Gadus chalcogrammus |  |
| N-tosyl-L-phenyl-alanine-chloromethyl ketone | 11% inhibition at 1mg/ml | Gadus chalcogrammus | |
| Soybean trypsin inhibitor | 91% inhibition at 1mg/ml | Gadus chalcogrammus |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | 10 mM Ca2+ have a stabilizing effect | Gadus chalcogrammus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 24000 | - |
SDS-PAGE | Gadus chalcogrammus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Gadus chalcogrammus | - |
walleye pollock | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| Sephacryl S-200 gel filtration and Sephadex G-50 gel filtration | Gadus chalcogrammus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| pyloric cecum | - |
Gadus chalcogrammus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.6 | - |
crude enzyme | Gadus chalcogrammus |
| 50 | - |
after 83fold putrification | Gadus chalcogrammus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| N-alpha-p-tosyl-L-arginine methyl ester + H2O | - |
Gadus chalcogrammus | N-alpha-p-tosyl-L-arginine + methanol | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
around 50°C | Gadus chalcogrammus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
unstable above 30°C | Gadus chalcogrammus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
around pH 8.0 | Gadus chalcogrammus |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 5 | - |
unstable below pH 5.0 | Gadus chalcogrammus |
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Release 2023.1 (January 2023)
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