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Literature summary for 3.4.21.4 extracted from

  • Gombos, L.; Kardos, J.; Patthy, A.; Medveczky, P.; Szilagyi, L.; Malnasi-Csizmadia, A.; Graf, L.
    Probing conformational plasticity of the activation domain of trypsin: the role of glycine hinges (2008), Biochemistry, 47, 1675-1684.
    View publication on PubMed
Search for more literature for 3.4.21.4

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli strain BL21(DE3)pLysS Homo sapiens

Protein Variants

Protein Variants Comment Organism
R193A slightly reduced activity Homo sapiens
R193G completely resistant to chymotryptic degradation Homo sapiens
R193G/G142A reduced activity Homo sapiens
R193G/G184A reduced activity Homo sapiens
R193G/G19A reduced activity, completely resistant to chymotryptic degradation Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
additional information trypsin 4 is completely resistant to chymotryptic degradation Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0141
-
 Z-Gly-Pro-Arg-p-nitroanilide mutant enzyme R193G/G142A, in 20 mM Tricine,10 mM CaCl2, at pH 8.0 and 20°C Homo sapiens
0.0203
-
 Z-Gly-Pro-Arg-p-nitroanilide mutant enzyme R193G, in 20 mM Tricine,10 mM CaCl2, at pH 8.0 and 20°C Homo sapiens
0.0266
-
 Z-Gly-Pro-Arg-p-nitroanilide mutant enzyme R193A, in 20 mM Tricine,10 mM CaCl2, at pH 8.0 and 20°C Homo sapiens
0.0298
-
 Z-Gly-Pro-Arg-p-nitroanilide wild type enzyme, in 20 mM Tricine,10 mM CaCl2, at pH 8.0 and 20°C Homo sapiens
0.0788
-
 Z-Gly-Pro-Arg-p-nitroanilide mutant enzyme R193G/G19A, in 20 mM Tricine,10 mM CaCl2, at pH 8.0 and 20°C Homo sapiens
0.346
-
 Z-Gly-Pro-Arg-p-nitroanilide mutant enzyme R193G/G184A, in 20 mM Tricine,10 mM CaCl2, at pH 8.0 and 20°C Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Q Sepharose Fast Flow column chromatography and Phenyl Sepharose Fast Flow column chromatography Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-methylumbelliferyl-4-guanidinobenzoate + H2O
-
 Homo sapiens 4-methylumbelliferol + 4-guanidinobenzoate
-
 ?
Z-Gly-Pro-Arg-p-nitroanilide + H2O
-
 Homo sapiens Z-Gly-Pro-Arg + p-nitroaniline
-
 ?

Synonyms

Synonyms Comment Organism
trypsin 4
-
 Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.86
-
 Z-Gly-Pro-Arg-p-nitroanilide mutant enzyme R193G/G142A, in 20 mM Tricine,10 mM CaCl2, at pH 8.0 and 20°C Homo sapiens
20 50 Z-Gly-Pro-Arg-p-nitroanilide wild type enzyme, in 20 mM Tricine,10 mM CaCl2, at pH 8.0 and 20°C Homo sapiens
29.2
-
 Z-Gly-Pro-Arg-p-nitroanilide mutant enzyme R193G/G184A, in 20 mM Tricine,10 mM CaCl2, at pH 8.0 and 20°C Homo sapiens
51.5
-
 Z-Gly-Pro-Arg-p-nitroanilide mutant enzyme R193G/G19A, in 20 mM Tricine,10 mM CaCl2, at pH 8.0 and 20°C Homo sapiens
54.1
-
 Z-Gly-Pro-Arg-p-nitroanilide mutant enzyme R193G, in 20 mM Tricine,10 mM CaCl2, at pH 8.0 and 20°C Homo sapiens
83.6
-
 Z-Gly-Pro-Arg-p-nitroanilide mutant enzyme R193A, in 20 mM Tricine,10 mM CaCl2, at pH 8.0 and 20°C Homo sapiens
88.2
-
 Z-Gly-Pro-Arg-p-nitroanilide wild type enzyme, in 20 mM Tricine,10 mM CaCl2, at pH 8.0 and 20°C Homo sapiens