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Literature summary for 3.4.21.4 extracted from

  • Zablotna, E.; Dysasz, H.; Lesner, A.; Jaskiewicz, A.; Kazmierczak, K.; Miecznikowska, H.; Rolka, K.
    A simple method for selection of trypsin chromogenic substrates using combinatorial chemistry approach (2004), Biochem. Biophys. Res. Commun., 319, 185-188.
    View publication on PubMed
Search for more literature for 3.4.21.4

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.03
-
 L-Phe-L-Val-L-Ile-N-(3-carbamoyl-4-nitrophenyl)-L-Arg-amide
-
 Bos taurus
0.044
-
 L-Phe-L-Val-L-Pro-N-(3-carbamoyl-4-nitrophenyl)-L-Arg-amide
-
 Bos taurus
0.096
-
 L-Phe-L-Val-L-Ile-N-(3-carbamoyl-4-nitrophenyl)-L-Lys-amide
-
 Bos taurus
0.158
-
 N-benzoyl-DL-Arg-4-nitroanilide
-
 Bos taurus
0.24
-
 L-Phe-L-Val-L-Pro-N-(3-carbamoyl-4-nitrophenyl)-L-Lys-amide
-
 Bos taurus

Organism

Organism UniProt Comment Textmining
Bos taurus
-
 isoform beta-trypsin
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-Phe-L-Val-L-Ile-(3-carbamoyl-4-nitrophenyl)-L-Arg-amide + H2O
-
 Bos taurus L-Phe-L-Val-L-Ile-L-Arg + 5-amino-2-nitrobenzamide
-
 ?
L-Phe-L-Val-L-Ile-N-(3-carbamoyl-4-nitrophenyl)-L-Arg-amide + H2O
-
 Bos taurus L-Phe-L-Val-L-Ile-L-Arg + 5-amino-2-nitrobenzamide
-
 ?
L-Phe-L-Val-L-Ile-N-(3-carbamoyl-4-nitrophenyl)-L-Lys-amide + H2O
-
 Bos taurus L-Phe-L-Val-L-Ile-L-Lys + 5-amino-2-nitrobenzamide
-
 ?
L-Phe-L-Val-L-Pro-N-(3-carbamoyl-4-nitrophenyl)-L-Arg-amide + H2O
-
 Bos taurus L-Phe-L-Val-L-Pro-L-Arg + 5-amino-2-nitrobenzamide
-
 ?
L-Phe-L-Val-L-Pro-N-(3-carbamoyl-4-nitrophenyl)-L-Lys-amide + H2O
-
 Bos taurus L-Phe-L-Val-L-Pro-L-Lys + 5-amino-2-nitrobenzamide
-
 ?
additional information strong interaction of Phe in position P4 with enzyme Bos taurus ?
-
 ?
N-benzoyl-DL-Arg-4-nitroanilide + H2O
-
 Bos taurus N-benzoyl-L-Arg + 4-nitroaniline
-
 ?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.8
-
 N-benzoyl-DL-Arg-4-nitroanilide
-
 Bos taurus
3.2
-
 L-Phe-L-Val-L-Ile-(3-carbamoyl-4-nitrophenyl)-L-Arg-amide
-
 Bos taurus
4
-
 L-Phe-L-Val-L-Pro-N-(3-carbamoyl-4-nitrophenyl)-L-Lys-amide
-
 Bos taurus
4.4
-
 L-Phe-L-Val-L-Ile-N-(3-carbamoyl-4-nitrophenyl)-L-Arg-amide
-
 Bos taurus
27.4
-
 L-Phe-L-Val-L-Pro-N-(3-carbamoyl-4-nitrophenyl)-L-Arg-amide
-
 Bos taurus